The effect of NDGA-modified etchant on the enzymatic degradation resistance and mechanical properties of collagen matrix  

作　　者：Shi-Qiang Gong Zhen-Jie Xue Shu-Teng Liao Ya-Bo Wu Yan Liu 

机构地区：[1]Center of Stomatology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology [2]Beijing National Laboratory for Molecular Science, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, the Chinese Academy of Sciences [3]Laboratory of Biomimetic Nanomaterials, Department of Orthodontics, Peking University School and Hospital of Stomatology, National Engineering Laboratory for Digital and Material Technology of Stomatology, Beijing Key Laboratory of Digital Stomatology

出　　处：《Chinese Chemical Letters》2018年第1期205-208,共4页中国化学快报（英文版）

基　　金：supported by the Projects of the National Natural Science Foundation of China(No. 81571815 (Y. Liu),No. 81401525 (S.Q. Gong));the Beijing Municipal Natural Science Foundation(No. 7152156 (Y. Liu));Beijing New-star Plan of Science and Technology(No. Z171100001117018 (Y. Liu))

摘　　要：Enzymatic degradation of demineralized collagen matrix seriously impairs durable resin-dentin bonding. In this study, we evaluated the effect of nordihydroguaiaretic acid （NDGA）-modified etchant on the resistance to enzymatic degradation and mechanical properties of demineralized collagen matrix. Dentin beams were randomly demineralized by following solutions： 1 ） 10% phosphoric acid （PhA） solution, 2） 10% dimethyl sulfoxide （DMSO）-PhA solution, 3） NDGA-modified etchant, and 4） proanthocyanidins （PA）-modified etchant. The demineralized dentin collagen was then digested by type l collagenase solution. The collagenase degradation resistance was evaluated by measuring loss of dry mass, hydroxyproline release, and elastic modulus change. The degradation rate and hydroxyproline release of dentin collagen treated with NDGA-modified etchant were significantly lower than those in the other groups （P〈 0.001）. The elastic modulus of dentin beams treated with NDGA-modified etchant did not increase significantly. However, after 48 h of collagenase degradation, the loss of elastic modulus of dentin beams treated with NDGA-modified etchant was significantly lower than that of the control group （P〈 0.001）. The NDGA-modified etchant could improve the resistance to enzymatic degradation of type l collagenase degradation, and the stability of the mechanical properties of dentin collagen. This proof-of-concept study validates the etch-and-crosslink technique that improves durability of dentin bonding through simultaneous dentin etching and collagen crosslinking.Enzymatic degradation of demineralized collagen matrix seriously impairs durable resin-dentin bonding. In this study, we evaluated the effect of nordihydroguaiaretic acid （NDGA）-modified etchant on the resistance to enzymatic degradation and mechanical properties of demineralized collagen matrix. Dentin beams were randomly demineralized by following solutions： 1 ） 10% phosphoric acid （PhA） solution, 2） 10% dimethyl sulfoxide （DMSO）-PhA solution, 3） NDGA-modified etchant, and 4） proanthocyanidins （PA）-modified etchant. The demineralized dentin collagen was then digested by type l collagenase solution. The collagenase degradation resistance was evaluated by measuring loss of dry mass, hydroxyproline release, and elastic modulus change. The degradation rate and hydroxyproline release of dentin collagen treated with NDGA-modified etchant were significantly lower than those in the other groups （P〈 0.001）. The elastic modulus of dentin beams treated with NDGA-modified etchant did not increase significantly. However, after 48 h of collagenase degradation, the loss of elastic modulus of dentin beams treated with NDGA-modified etchant was significantly lower than that of the control group （P〈 0.001）. The NDGA-modified etchant could improve the resistance to enzymatic degradation of type l collagenase degradation, and the stability of the mechanical properties of dentin collagen. This proof-of-concept study validates the etch-and-crosslink technique that improves durability of dentin bonding through simultaneous dentin etching and collagen crosslinking.

关 键 词：NDGACrosslinkDegradationMechanical propertiesCollagen 

分 类 号：O6[理学—化学]