猪源解鸟氨酸拉乌尔菌分离鉴定与大环内酯耐药基因检测  

Isolation and identification of Raoultella ornithinolytica and detection of macrolide resistance gene

作　　者：田佳琪[1] 董文龙[1] 王巍[1] 王羽[1] 张宏伟[1] 张喜庆[1] 李雪嵩 马红霞[1] 高云航[1] 

机构地区：[1]吉林农业大学动物科学技术学院,吉林长春130118

出　　处：《中国预防兽医学报》2018年第1期1-4,共4页Chinese Journal of Preventive Veterinary Medicine

基　　金：吉林省现代农业产业技术体系建设专项(2015-24);吉林省动物疾病防治研究创新团队(20111820)

摘　　要：为确定吉林省某养殖场导致猪群死亡的病原菌,本研究从病死猪胸腔积液中分离得到一株优势菌,经16S r DNA检测该株优势菌为解鸟氨酸拉乌尔菌;动物回归试验显示分离株对猪、小鼠均有致病性;药敏试验结果显示该菌对大环内酯类的麦迪霉素、阿奇霉素、红霉素、泰乐菌素呈显著耐药,并通过PCR检测到分离菌株的大环内酯耐药基因呈显性表达。本研究首次从猪体内分离到解鸟氨酸拉乌尔菌,并为该条件致病菌的临床诊疗、免疫防控提供参考依据。To investigate the pathogenic bacteria caused the swine dead in Jilin provience, the dominant bacteria was isolated from pleural effusion, and detected as Raoultella orithinolytica by I6S rDNA PCR amplification and sequencing. The result of animal regression experiment showed the isolate had pathogenicity to swine and mice. The result of minimum inhibitory concentration （MIC） showed that the isolated bacteria was resistant to maddie azithromycin, erythromycin and tylosin, and macrocyclic resistant gene of Raoultella orithinolytica was detected by PCR. The study was the first time to isolate Raoultella orithinolytica from swine, and the experiment data could provide the reference basis for clinical treatment and immune prevention of the conditional pathogen.

关 键 词：解鸟氨酸拉乌尔菌 分离鉴定 药敏试验 大环内酯耐药基因 

分 类 号：S852.4[农业科学—基础兽医学]