1,3-丁二烯生物接触限值研究  

Biological exposure limit of 1,3-butadiene occupational exposure

作　　者：孔凡玲[1] 周景洋[1] 赵敬[2] 李仁波[1] 程学美[1] 

机构地区：[1]山东省疾病预防控制中心,山东济南250014 [2]山东省职业卫生与职业病防治研究院,250062

出　　处：《中国职业医学》2017年第6期721-724,共4页China Occupational Medicine

基　　金：国家职业卫生标准项目(20130202);山东省自然科学基金(ZR2010HQ042);山东省医药卫生研究项目(2009HZ050);山东省医药卫生科技发展计划项目(2015WS0275);山东省中医药科技发展计划项目(2015-317)

摘　　要：目的制定我国1,3-丁二烯接触工人的生物接触限值。方法采用判断抽样方法,选择某合成橡胶厂139名1,3-丁二烯作业工人为接触组,另选择无职业性1,3-丁二烯接触的45名工作人员为对照组。采用气相色谱-火焰离子化检测法测定工作场所空气中1,3-丁二烯水平,以超高效液相色谱-质谱/质谱法测定2组工人班末尿中1,2-双羟基-4-(N-乙酰半胱氨酸)-丁烷(DHBMA)水平,分析两者的相关性,推算1,3-丁二烯的生物接触限值。结果接触组工人接触1,3-丁二烯的时间加权平均浓度(C_(TWA))为0.004~7.609 mg/m^3,中位数(M)为0.253mg/m^3,班末尿中DHBMA水平为0.171~4.235 mg/g肌酐,M为1.220 mg/g肌酐;对照组工人接触1,3-丁二烯的C_(TWA)低于检出限,班末尿中DHBMA水平为0.157~1.808 mg/g肌酐,M为0.627 mg/g肌酐。接触组工人班末尿中DHBMA水平高于对照组(P<0.01)。接触组工人班末尿中DHBMA水平(y)与工作场所空气中1,3-丁二烯水平(x)呈正相关(y=0.349 x+1.082,P<0.01)。结论班末尿中DHBMA可作为1,3-丁二烯接触的生物标志物;建议将其生物接触限值定为2.900 mg/g肌酐。Objective To study the biological exposure limit of 1,3-butadiene occupational exposure. Methods A total of 139 workers who exposed to 1,3-butadiene in a rubber plant was chosen as exposure group, and 45 workers without 1,3- butadiene exposure were chosen as control group by judgment sampling method. Gas chromatography-flame ionization detection method was used to detect the level of 1, 3-butadiene in workplace air, and ultra-high performance liquid chromatography-tandem mass spectrometric was used to determine the level of urinary dihydroxybutyl mercapturic acid （DHBMA） in end-of-shift workers. The correlation between 1,3-butadiene level and urinary DHBMA level was analyzed. The biological limit of 1,3-butadiene occupational exposure was assessed. Results The range of concentration-time weighted average （CTWA） of 1, 3-butadiene was from 0. 004 to 7. 609 mg/m^3, the medium was 0. 253 mg/m^3 in the exposure group, while the level of urinary DHBMA was from 0. 171 to 4. 235 mg/g Cr, and the medium was 1. 220 mg/g Cr. The range of CTWg of 1,3-butadiene was below detection limit in the control group. The level of urinary DHBMA was from 0. 157 to 1. 808 mg/g Cr, and the medium was 0. 627 mg/g Cr in the control group. The level of urinary DHBMA in the exposure group was higher than that of the control group （P 〈 0. 01 ）. The level of urinary DHBMA （y） of workers was related to the level of 1,3-butadiene （x） of the workplace air in the exposure group （y = 0. 349 x ＋ 1. 082, P 〈 0. 01 ）. Conclusion DHBMA in urine could be used as a biomarker for 1,3-butadiene exposure in workers. The recommended biological exposure limit of DHBMA is 2. 900 mg/g Cr.

关 键 词：1 3-丁二烯 1 2-双羟基-4-(N-乙酰半胱氨酸)-丁烷 生物标志物 职业接触 生物监测 生物接触限值 

分 类 号：R135[医药卫生—劳动卫生]