高效液相色谱法检测饮用水中吐纳麝香  

Detection of Tonalide in Drinking Water Using High Performance Liquid Chromatography

作　　者：邵煜[1] 孙哲 余永强 王丽丽 李建 陈蓉 张土乔[1] 刘小为 

机构地区：[1]浙江大学建筑工程学院市政工程研究所,浙江杭州310058 [2]浙江农林大学暨阳学院生物环境系,浙江诸暨311800

出　　处：《中国给水排水》2018年第2期112-117,共6页China Water & Wastewater

基　　金：国家自然科学基金资助项目(51408539;51208457;51478417)

摘　　要：多环麝香类物质吐纳麝香(AHTN)是近年来在饮用水及饮用水源中检出频率较高的一种新型污染物。目前普遍采用气相色谱质谱联用(GC-MS)方法检测水中AHTN。针对GCMS方法存在的一些不足,开发了一种简单、快速的高效液相色谱(HPLC)测定方法。新的HPLC测定方法能在5 min内完成样品的检测,辅以固相萃取样品富集技术,检测限可达ng/L水平。新方法将水样与乙腈以一定比例混合,明显改善了AHTN在液相色谱柱中的分离效果并消除了滤膜对样品回收率的影响。对GC-MS法和HPLC法进行对比试验发现,两种方法测定同一样品(纯水和实际水体本底)时相对误差≤2.0%,二者之间的相对偏差<1.0%。因此,HPLC法可作为检测水中AHTN的备选方法。该方法对水相中溶解度较小、极性较弱的其他有机物的检测具有借鉴意义。Polycyclic quently detected in drinking musk tonalide （AHTN） is an emerging organic pollutant which has been fre- water and source water in recent years. The gas chromatography-mass spec- trometry （GC-MS） is usually used as the detection method for AHTN. While, there are some problems for GC-MS. So a simple and rapid high performance liquid chromatography （HPLC） method was devel- oped to overcome the deficiency of GC-MS. The sample analysis could be completed with HPLC method within 5 rain. Assisted with enrichment techniques such as solid phase extraction, the detection limit canreach to ng/L level. In the new detection process, aqueous AHTN sample is mixed with acetonitrile in a certain ratio, which improves the separation efficiently of AHTN in liquid chromatography column and eliminate the influence of membrane filtration on the recovery of samples. By comparison, when samples prepared with pure water and actual water were determined, it was found that the relative errors for the two methods were less than 2.0% and the relative deviation of the two methods was less than 1.0%. Thus, the developed HPLC method can be used as an alternative method for detecting AHTN in water. This method provides reference to the determination of other organic compounds with low water solubility and weak polarity.

关 键 词：饮用水 吐纳麝香 高效液相色谱法 参数优化 

分 类 号：TU991[建筑科学—市政工程]