CO_2浓度对雨生红球藻生理生化指标的影响  

Effects of CO_2 Concentrations on the Physiological and Biochemical Parameters of Haematococcus pluvialis

作　　者：陈佳妮 林丽春[1] 徐年军[1] 张琳[1] 孙雪[1] 

机构地区：[1]宁波大学海洋学院,宁波315211

出　　处：《生物技术通报》2018年第1期239-246,共8页Biotechnology Bulletin

基　　金：国家自然科学基金项目(31572638);浙江省科技厅公益项目(2015C32021);宁波市科技计划项目(2014C10023)

摘　　要：为了探讨雨生红球藻对不同CO_2浓度的响应,利用生理生化测定方法比较了两种CO_2浓度对雨生红球藻生长、色素含量、叶绿素荧光参数和两种碳代谢相关酶的影响。结果显示在雨生红球藻的绿色营养阶段,4倍空气CO_2浓度下培养的藻细胞密度是空气CO_2浓度下的3.08倍(第10天),与空气CO_2相比,较高CO_2培养藻的叶绿素和类胡萝卜素含量、胞外碳酸酐酶(CA)活性降低,非光化学淬灭(NPQ)显著升高,最大光能转化效率(Fv/Fm)、实际光化学量子效率(ФPSII)多显著升高,而光化学淬灭(q P)、核酮糖-1,5-二磷酸羧化酶/加氧酶(Rubisco)活性无显著差异。在雨生红球藻的红色孢子阶段,4倍空气CO_2培养下的藻细胞密度显著降低,但虾青素含量提高了20.23%(第8天)。以上研究表明可以通过适当提高CO_2浓度来促进雨生红球藻的生长和虾青素的积累。In order to explore the response of microalga Haematococcus pluvialis to different CO2 concentrations, the effects of two kinds of CO2 concentrations on the algal growth, pigment content, chlorophyll fluorescence parameter and activities of two carbon metabolism-related enzymes, were investigated by the physiological and biochemical methods. Results showed that at the green stage, compared to the air CO2, the growth rate of H. pluvialis increased 3.08 times （ 10 th ） under quadruple air CO2 condition ; the chlorophyll and carotenoid content, and the extracellular carbonic anhydrase activity all decreased ; non-photochemical quenching （ NPQ ）, most of quantum yield of photo-chemical electron transport ~ ~ PSlJ ）, and maximal photo-chemical efficiency of PSⅡ （ Fv8Fm ） almost significantly increased, whereas the photo-chemical quenching （qP） and activity of ribulose-1, 5-bisphosphate earboxylase/oxygenase （Rubisco） differed insignificantly. At the red stage, the algae cultured with quadruple air CO2 grew slower, however, their astaxanthin content increased by 20.23% （ 8 d ） of that under air CO2 condition. The study indicated that appropriately high CO2 concentration promoted its growth rate and astaxanthin accumulation in H.pluvialis.

关 键 词：雨生红球藻 CO2 藻细胞密度 色素含量 叶绿素荧光参数 

分 类 号：Q945[生物学—植物学]