热休克蛋白70与汉坦病毒持续性感染乳大鼠大脑皮层星形胶质细胞(英文)  

Heat shock protein 70 and Hantavirus persistent infection of cortical astrocytes from newborn rats

作　　者：贺帅 杨守京[2] 

机构地区：[1]包头医学院第一附属医院病理科包头医学院病理学教研室,内蒙古包头014010 [2]第四军医大学病理学教研室,西安710032

出　　处：《解剖学报》2018年第1期7-13,共7页Acta Anatomica Sinica

基　　金：Supported by National Natural Science grants 30271186

摘　　要：目的探讨热休克蛋白70(HSP70)过表达可能有助于汉坦病毒(HTNV)持续感染。方法在HTNV持续性感染过程中,通过Westen blotting及RT-PCR检测大脑皮层星形胶质细胞中HSP70的表达情况,并通过免疫荧光显微技术、Annexin V及RT-PCR检测细胞凋亡。结果与对照组相比,感染HTNV 76-118或SEOV L99的星形胶质细胞HSP70蛋白的表达增加,且在HTNV 76-118感染后3d或SEOV L99感染后2d HSP70蛋白表达达到峰值(n=15,P<0.01,P<0.05);感染HTNV 76-118及SEOV L99上调星形胶质细胞HSP70基因的表达,且在HTNV76-118感染后2~5d或SEOV L99感染后2~4d HSP70基因表达达到峰值(n=15,P<0.01,P<0.05)。HTNV76-118或SEOV L99感染过程中,星形胶质细胞无细胞病变效应,并且病毒复制未诱导细胞凋亡。结论 HTNV感染星形胶质细胞激活了HSP70,这种改变可能抑制细胞凋亡,促进病毒持续性感染。Objective To define that heat shock protein 70（ HSP70） overpression might contributed to the persistent infection of Hantaviruses（ HTNV）. Methods The expression of HSP70 in cortical astrocytes was examined by Western blotting and RT-PCR during Hantaviruses persistent infection. The following apoptosis was detected by immunofluorescence microscopy,Annexin V assays and RT-PCR assay. Results Compared to control group,the expression of HSP70 protein in astrocytes infected with HTNV 76-118 or SEOV L99 increased,peak expression of HSP70 at day 3 following HTNV 76-118 infection or at day 2 following SEOV infection（ n = 15,P 〈 0. 01,P 〈 0. 05）,and the expression of HSP70 gene in astrocytes infected with HTNV 76-118 or SEOV L99 up-regulated,peak expression of HSP70 at day 2-5 post HTNV76-118 infection and at day 2-4 post SEOV L99 infection（ n = 15,P 〈 0. 01,P 〈 0. 05）. In the course of HTNV76-118 or SEOV L99 infection,any cytopathic effect was not shown in astrocytes and cell apoptosis was not induced by viral replication. Conclusion HSP70 induced by Hantaviruses infection might inhibit apoptosis and contribute to the viral persistent infection.

关 键 词：汉坦病毒 大脑皮层 星形胶质细胞 热休克蛋白70 免疫印迹法 反转录-聚合酶链反应 大鼠 

分 类 号：R373.32[医药卫生—病原生物学]