游离态四环素类抗生素抗性基因在土壤中的吸附  

Soil Adsorption Characteristics of Extracellular Tetracyclines Antibiotic Resistance Genes

作　　者：刘博[1,2] 周方 刘翔[1] 李淼[1] 

机构地区：[1]清华大学环境学院,北京100084 [2]国网山东省电力公司经济技术研究院,山东济南251000

出　　处：《中国给水排水》2018年第3期91-96,共6页China Water & Wastewater

基　　金：国家自然科学基金资助项目(51378287)

摘　　要：针对地下水抗生素抗性基因的来源和传播,结合北京再生水入渗现场四环素类抗生素抗性基因的分布特征,以p UC18质粒作为载体,分别导入tet B、tet M、tet O和tet W基因片段作为模式抗性基因,分析了游离态四环素类抗生素抗性基因在土壤中的吸附规律。结果表明,对于四环素类抗生素抗性基因,不同目的片段对其在包气带土壤介质中的平衡吸附量没有影响,而底泥对携带tet O和tet W目的片段的质粒吸附量相对较高。In this study, tetracyclines antibiotic resistance genes using pUC18 plasmids （pattern resistant genes were tetB, terM, tetO and tetW） as carriers were considered as the targeted pollutants, and the adsorption and transformation characteristics from a reclaimed water infiltration site in Beijing were analyzed in the batch and column experiments. The results revealed that the adsorption of the plasmids with different target genes was similar in the vadose zone, whereas the adsorptions of tetO and tetW genes were higher in sediment soil.

关 键 词：四环素类抗生素抗性基因 质粒 吸附特性 土壤 

分 类 号：TU992[建筑科学—市政工程]