高效合相色谱同时测定血浆中12种磺胺类药物  

Determination of 12 sulfonamides in plasma by ultra performance convergence chromatography

作　　者：张元 闫加庆[1] 刘敏 张远远 刘佳 李国辉 张峰 

机构地区：[1]国家癌症中心/中国医学科学院北京协和医学院肿瘤医院药剂科,北京100021 [2]中国检验检疫科学研究院食品安全研究所,北京100176

出　　处：《分析试验室》2018年第2期152-156,共5页Chinese Journal of Analysis Laboratory

基　　金：中国医学科学院医学与健康科技创新工程(2016-I2M-1-001)资助

摘　　要：建立了一种同时分离测定血浆中12种磺胺类药物的高效合相色谱(Ultra Performance Convergence Chromatography,UPC^2)方法。血浆样品中加入乙腈,涡旋混合后离心,上清液过滤后采用UPC^2方法测定,采用Acquity UPC^(2TM)BEH色谱柱,CO_2和甲醇作为流动相,梯度洗脱,柱温箱温度40℃,系统背压14.5MPa。该法在6 min内实现了对12种磺胺类药物的基线分离,定量限为0.5μg/mL,回收率在86.2%~99.5%之间。方法适用于血浆中多种磺胺类药物的同时检测。An analytical method based on UPC^2 has been developed to quantity 12 suitonamides m plasma. Plasma samples were added with acetonitrile, then the samples were vortexed and centrifuged. The supernatant was filtered and analyzed using UPC^2. Acquity UPC^2TM BEH column was used as the separation column. CO2 and methanol were used as mobile phase, the column oven temperature was 40℃, and the backpressure was 14.5MPa. The method allowed the determination of 12 sulfonamides and metabolites in plsama samples less than 6 vain. The limit of quantification was 0. 5 μg/mL. Recoveries between 86.2% and 99.5% were obtained. The method is simple, accurate, time-saving and more environmen-friendly, and it is applicable to a variety of sulfonamides detection.

关 键 词：合相色谱 磺胺 代谢物 分离. 

分 类 号：R446[医药卫生—诊断学] O657.7[医药卫生—临床医学]