抗菌肽Sublancin增强小鼠获得性免疫的研究  

Study of Antimicrobial Peptide Sublancin Enhance Acquired Immunity of Mice

作　　者：张晓雅 杨青[1] 王帅 杨天任 曾祥芳[1] 谯仕彦[1] 

机构地区：[1]中国农业大学动物科技学院,北京100193

出　　处：《动物营养学报》2018年第1期236-245,共10页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：国家重点研发计划课题"新型饲用抗生素替代品及其综合应用技术研究"(2016YFD0501308)

摘　　要：本试验旨在研究抗菌肽Sublancin灌胃对卵清白蛋白(OVA)免疫小鼠获得性免疫反应的影响。试验选用6周龄雌性BALA/c小鼠60只,随机分为5个组,每组12只小鼠。空白对照组小鼠灌胃生理盐水,阳性对照组小鼠灌胃2.5 mg/kg体重的左旋咪唑溶液,抗菌肽Sublancin组小鼠灌胃0.5、1.0和2.0 mg/kg体重的抗菌肽Sublancin溶液。各组小鼠连续灌胃14 d。在灌胃结束24 h后用模式抗原OVA皮下注射对各组小鼠进行免疫,14 d后加强免疫1次,二次免疫7 d后采血和取脾脏,用间接酶联免疫吸附测定(ELISA)法对血清OVA特异性免疫球蛋白G(Ig G)及其亚类Ig G1、Ig G2a和细胞因子含量进行检测。结果表明:1)与空白对照组相比,0.5、1.0和2.0 mg/kg抗菌肽Sublancin组和阳性对照组小鼠血清OVA特异性Ig G含量均显著或极显著提高(P<0.05或P<0.01)。2)与空白对照组相比,1.0和2.0 mg/kg抗菌肽Sublancin组和阳性对照组小鼠血清OVA特异性Ig G1含量显著或极显著提高(P<0.05或P<0.01),1.0 mg/kg抗菌肽Sublancin和阳性对照组小鼠血清OVA特异性Ig G2a含量显著提高(P<0.05),1.0 mg/kg抗菌肽Sublancin组和阳性对照组的小鼠脾脏淋巴细胞的刺激指数显著提高(P<0.05)。3)与空白对照组相比,1.0 mg/kg抗菌肽Sublancin组和阳性对照组的小鼠脾脏淋巴细胞上清液中1型辅助性T细胞(Th1)细胞因子干扰素-γ(IFN-γ)和白细胞介素-2(IL-2)的含量均显著或极显著提高(P<0.05或P<0.01)。4)与空白对照组相比,0.5和1.0 mg/kg抗菌肽Sublancin组和阳性对照组的小鼠脾脏淋巴细胞上清液中2型辅助性T细胞(Th2)细胞因子白细胞介素-4(IL-4)和白细胞介素-10(IL-10)的含量均显著或极显提高(P<0.05或P<0.01)。5)0.5、1.0和2.0 mg/kg抗菌肽Sublancin组与阳性对照组之间的以上检测指标均没有显著差异(P>0.05)。本试验结果表明,抗菌肽Sublancin可以诱导OVA免疫小鼠产生Th1和Th2混合型免疫反应,增强其体液免疫和细胞免疫功能。This experiment was designed to investigate the effects of intragastric administration of antimicrobial peptide Sublancin on acquired immunity of mice immunized with ovalbumin（ OVA）. Sixty BALB/c mice were randomly assigned into 5 groups with 12 mice per group. Mice in the blank control group received normal saline by intragastric administration,mice in the positive control group received 2.5 mg/kg body weight（ BW）levamisole by intragastric administration,and mice in the antimicrobial peptide Sublancin groups received 0.5,1.0 and 2. 0 mg/kg BW antimicrobial peptide Sublancin by intragastric administration,respectively. All the mice were administrated intragastric daily for 14 days. Mice were immunized with model antigen OVA by subcutaneous infection after the intragastric administration 24 h,and enhanced immunization 1 time after 14 days,took blood and spleen after the second immunization 7 days,the contents of OVA-specific immunoglobulin（ IgG） and its ubtype IgG1,IgG2 a and cytokine in serum were detected by indirect enzyme linked immunosorbent assay（ ELISA） method. The results showed as follows： 1） compared with the blank control group,the serum OVA-specific IgG content of mice in 0.5,1.0 and 2.0 mg/kg antimicrobial peptide Sublancin groups and positive control group was significantly increased（ P0.05 or P0.01）. 2） Compared with the blank control group,the serum OVA-specific IgG1 content of mice in 1.0 and 2.0 mg/kg antimicrobial peptide Sublancin groups and positive control group was significantly increased（ P0.05 or P0.01）,the serum OVA-specific IgG2 a content of mice in 1.0 mg/kg antimicrobial peptide Sublancin group and positive control group was significantly increased（ P0.05）,the spleen lymphocytes stimulation index of mice in 1.0 mg/kg antimicrobial peptide Sublancin group and positive control group was significantly increased（ P 0. 05）. 3） Compared with the blank control group,the contents of T helper type 1 cell（ Th1） interferon-γ（ IFN-γ） and interleuki

关 键 词：抗菌肽 Sublancin 小鼠 体液免疫 细胞免疫 

分 类 号：S852.4[农业科学—基础兽医学]