小鼠睾丸组织中雄激素及其受体信号抑制精母细胞干扰素刺激因子15基因表达  

Inhibitory Effect of Androgen and Its Receptor on the Expression of Interferon-stimulated Gene 15 in Mouse Spermatocytes

作　　者：王恒 潘莉[2] 郭晓强[2] 林穆奇[2] 陈欢 张巧霞 

机构地区：[1]广州医科大学研究生院,广州市511436 [2]深圳市第二人民医院/深圳大学第一附属医院,广东省深圳市518035

出　　处：《医学分子生物学杂志》2018年第1期13-16,共4页Journal of Medical Molecular Biology

基　　金：国家自然科学基金（No.81270686）,深圳市科技计划项目（No.JCYJ20150330102720163）,深圳骨髓移植技术临床应用公共服务平台.

摘　　要：目的在前期研究中进行全基因组表达谱检测发现在雄激素受体（androgenreceptor，Ar）基因睾丸支持细胞特异性敲除小鼠（s—Ar/y）睾丸组织中干扰素刺激因子15（interferon—stimulatedgene15,Isg15）基因表达增高。研究的目的是了解睾丸组织中雄激素及其受体对Isg15基因表达的影响作用。方法采用RT—qPCR和Western印迹等方法比较野生型小鼠和s—Ar由小鼠睾丸组织中Isg15基因表达量。通过免疫组织化学和荧光染色两种方法检测Isg15蛋白在睾丸组织中的分布。结果在s—Ar由小鼠睾丸组织中tsg15基因表达量比在野生型小鼠中显著升高，Isg15蛋白主要表达于精母细胞。结论睾丸支持细胞特异性敲除加基因使精母细胞中Isg15基因表达异常增高。Objective The expression of interferon-stimulated gene 15 （Isg15） was found to increase in the testes of Sertoli cell specific Ar knockout （S-Ar-/y） mice. This study aimed to exam- ine the effect of androgen and its receptor on the expression of Isg15. Methods RT-qPCR and Western blotting were used to detect the expression of Isgl5 in wide-type mice and S-Ar/y mice and the difference was compared. The distribution of Isg15 protein in mouse testis was measured by im- munohistochemistry and immunofluorescence. Results The expression of Isg15 gene was signifi- cantly higher in S-At-/y mice than that in wild-type mice, and the Isg15 protein was mainly expressed in spermatocytes. Conclusion Sertoli cell specific knockout of Ar gene Results in abnormally high expression of Isg15 gene in spermatocytes.

关 键 词：雄激素受体 干扰素刺激因子15 基因表达 

分 类 号：Q786[生物学—分子生物学]