补肾壮骨颗粒对SAMP6小鼠股骨宏观结构及生物力学的影响  

The effects of Bushen Zhuanggu Granules on macrostructure and biomechanical properties of the femur of SAMP6 mice

作　　者：汤璐璐 邓伟民[1,2] 苏海容 袁人飞[1,2] 

机构地区：[1]广东药科大学,广东广州510006 [2]广州军区广州总医院,广东广州510010 [3]广州中医药大学,广东广州510403

出　　处：《中国骨质疏松杂志》2018年第1期91-97,共7页Chinese Journal of Osteoporosis

基　　金：国家自然科学基金(81273778);军队保健专项项目(13BJZ13;16BJZ57)

摘　　要：目的研究中药复方补肾壮骨颗粒对SAMP6小鼠股骨结构及生物力学性能的影响,并探讨是否有可能替代生长激素对老年男性骨质疏松进行治疗。方法将30只3月龄SAMP6小鼠随机均分为模型组、生长激素组和中药组,选取10只正常老化小鼠(SAMR1)作为同源对照组。正常饲养1周后,生长激素组和中药组小鼠开始给药,每周称重1次,调整给药剂量,正常组和模型组给予等量的生理盐水。3个月后每组随机选取5只小鼠去眼球取血处死,取其左侧股骨,进行测定股骨的骨量、几何尺寸、骨截面力学几何量和生物力学性能实验,6个月后采取同样的方法处理剩余的小鼠。结果与模型组比较,3个月后用药组骨量均降低(P>0.05),6个月后均升高(P>0.05),且中药组均比同期生长激素组骨量高(P>0.05);3个月后中药组SAMP6小鼠皮质骨厚度、截面积增大(P<0.05),骨髓腔直径、横截面积减小(P<0.05),骨截面力学几何量增加(P>0.05),骨结构力学各参数均显著性提高(P<0.01),最大应力、弹性应力显著性提高(P<0.01),刚性系数增大(P<0.05),弹性模量增加(P>0.05);6个月后中药组SAMP6小鼠皮质骨厚度、横截面积增大(P<0.01),骨髓腔直径、横截面积减小(P<0.05),骨的截面力学几何量均显著性提高(P<0.01),最大载荷、弹性载荷提高(P<0.05),最大绕度、最大弯矩、能量吸收提高(P>0.05),弹性应力、刚性系数增加(P>0.05),弹性模量降低(P>0.05);与生长激素组比较,3个月后中药组SAMP6小鼠各检测指标结果无明显差异(P>0.05),6个月后中药组SAMP6小鼠皮质骨厚度、横截面积显著性增大(P<0.01),骨截面力学几何量增加(P>0.05),骨生物力学性能参数结果减小,但无统计学意义(P>0.05)。结论补肾壮骨颗粒可以调节SAMP6小鼠骨宏观几何结构的力学应答机能,改善骨的生物力学性能,增加骨质韧性,增强骨强度,提高抵抗外力变形和骨折的能力;长期用药可提高SAMP6小鼠�Objective To study the effects of Bushen Zhuanggu Granules on structure and biomechanical properties of the femur of senescence accelerated male mice（SAMP6）,and to explore whether it would replace growth hormone for the treatment of osteoporosis in elderly men.Methods 30 senescence accelerated male mice（SAMP6） aged three mouths were divided randomly into model group,growth hormone group（GH） and chinese medicine group,10 normal accelerated male mice（SAMR1） with the same age were selected as homologous control group.After a week of normal feeding,the mice of GH group and chinese medicine group were given medicine,weighed once a week and we adjusted dose by the weight.The mice of model and normal groups were given equivalent normal saline.Then five mice in each group randomly selected were executed by collecting eyeball blood after 3 mouths and immediately took out the left femur for measuring the bone mass and femoral geometry size and mechanical geometry of bone section and biomechanics capabilities detection.Results Compared with the model group,bone mass decreased in the medication groups after 3 months（P〉0.05）,and increased after 6 months（P〉0.05）,and the bone mass of the chinese medicine group was higher than that of the growth hormone group over the same period（P〉0.05）; 3 months later,cortical bone thickness and sectional area increased（P〈0.05）,the diameter of the bone marrowcavity and the cross sectional area decreased（P〈0.05）,the mechanical geometry of bone section increased（P〉0.05）,the parameters of bone structure mechanics were significantly improved（P 〈0.01）,the maximum stress and elastic stress increased（P 〈0.01）,the stiffness coefficient increased（P〈0.05）,and the elastic modulus increased（P〈0.05） of the SAMP6 in chinese medicine group; 6 months later,the cortical bone thickness and cross-sectional area increased（P〈 0.01）,bone marrowcavity diameter and cross-sectional area decreased（P〈0.05）,the mechanical geometry of

关 键 词：中医中药 补肾壮骨颗粒 快速老化雄性小鼠 骨质疏松 骨生物力学 宏观结构 

分 类 号：R285.5[医药卫生—中药学] R332[医药卫生—中医学]