CD226分子对小鼠海马相关恐惧记忆的影响  

Effects of CD226 on hippocampus-related fear memory in mice

作　　者：金敬一 王宁[1] 曾汉玉 赵广超 解柔刚[3] 陈丽华[1] 

机构地区：[1]第四军医大学基础医学院免疫学教研室,西安710032 [2]第四军医大学附属西京医院麻醉科,西安710032 [3]第四军医大学基础医学院神经生物学教研室,西安710032

出　　处：《神经解剖学杂志》2018年第1期92-96,共5页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金(81571531)

摘　　要：目的:探讨CD226分子对C57BL/6小鼠恐惧记忆的影响。方法:对野生型(Wild type,WT)小鼠和CD226基因敲除(CD226 knockout,Cd226^(-/-))小鼠进行非伤害性声音刺激和/或不可逃避的足底电击刺激联合匹配训练建立场景性恐惧条件化和线索性恐惧条件化模型,分别检测此两种小鼠在不同恐惧条件化模型中的行为反应,利用僵立时间占总测试时间的百分比来评估小鼠的恐惧程度。结果:(1)在场景性恐惧记忆(即海马相关恐惧记忆)检测中,Cd226^(-/-)小鼠僵立时间占总测试时间百分比明显高于野生型小鼠(P=0.090)。(2)在线索性恐惧记忆(即非海马相关恐惧记忆)检测中,结果无显著差异和趋势(P=0.641)。结论:Cd226^(-/-)小鼠海马相关恐惧记忆能力有增强趋势,提示CD226分子可能参与了小鼠海马相关恐惧记忆的调节。Objective： To explore the effect of CD226 on fear memory in C57BL/6 mice. Mothords： To establish the contextual and cued fear conditioning test, CD226 knockout（ Cd226^-/- ）and wild-type（WT） rr ice were given an unconditioned stimulus（ an electric footshock）and a conditioned stimulus（ a praticular context or an auditory cue）, freezing responge during the test was measured as an index of fear memory. Results： There was no stalistical significance on contextual fear memory and cued fear memory between WT and Cd226^-/- mice, but compared with WT mice, Cd226^ -/- mice exhibited an enhanced trend of contextual fear memory. Conclusion： Our findings suggest that CD226 may affect the hippocampns-related fear memory in mice.

关 键 词：CD226 场景性恐惧记忆 线索性恐惧记忆 海马区 

分 类 号：R338[医药卫生—人体生理学]