IκBα转基因小鼠肝脏免疫细胞亚群的初步分析  

Analysis of immune cells subsets in liver from IκBα-transgenic mice

作　　者：王学耀 韩秋菊[1] 张建[1] 

机构地区：[1]山东大学药学院免疫药物学研究所,济南250012

出　　处：《中国免疫学杂志》2018年第1期11-14,24,共5页Chinese Journal of Immunology

基　　金：国家自然科学基金(30628014)资助项目

摘　　要：目的:分析IκBα转基因小鼠肝脏组织形态、免疫细胞分群、凋亡状态以及表型分子等。方法:HE组织染色分析野生型小鼠和IκBα转基因小鼠肝脏组织形态变化;利用流式细胞术分析野生型小鼠和IκBα转基因小鼠肝脏组织中NK、NKT、T细胞比例和表型分子,进一步采用Annexin V标记法检测小鼠肝脏免疫细胞的凋亡状态;定量PCR法检测趋化因子和细胞因子的表达含量。结果:与野生型小鼠相比,IκBα转基因鼠肝脏组织损伤严重;IκBα转基因小鼠肝脏NK、NKT、T细胞比例均显著下降,NK细胞凋亡的比例增加,并且NKp46在IκBα转基因小鼠肝脏NK细胞中表达降低。在此过程中,还观察到IκBα转基因小鼠肝脏细胞因子谱发生改变,IFN-γ、CCL2等表达显著下降,而IL-2、IL-15等并没有显著变化。结论:IκBα转基因小鼠肝脏组织形态、免疫细胞亚群比例、表型及细胞因子谱均发生改变。Objective： To analyze the liver injury,and the percentage,apoptotic status,cytokine profiles of immune cell subsets in liver from IκBα transgenic mice. Methods： HE staining was performed to detect the liver injury. FACS was used to evaluate the percentage and phenotype of immune cell subsets,including T cells,NK cells and NKT cells,and Annexin V staining was used to evaluate the apoptosis rate of NK and T cells. Furthermore,real-time quantitive PCR was performed to analyze the expression of CCL2,IFN-γ,IL-2 and IL-15. Results： Liver injury was observed in IκBα transgenic mice. The percentage of T cells was lower in liver from IκBαtransgenic mice than that in Wild Type（ WT） mice,a similar trend was found in NK cells and NKT cells. We also found that NKp46 was inhibited in NK cells from IκBα transgenic mice,accompanied with the increased apoptosis. Also,IFN-γ and CCL2 were decreased in IκBα transgenic mice. Conclusion： The percentage,phenotype and cytokine profile of immune cell subsets were affected in IκBα-transgenic mice.

关 键 词：肝脏免疫 IΚBΑ NF-ΚB 凋亡 

分 类 号：R446.61[医药卫生—诊断学]