IL-17参与巨细胞病毒肝炎致病机制的研究  

Investigation of pathogenesis of IL-17 involved in cytomegalovirus hepatitis

作　　者：刘玲玲[1] 黄媛[1] 马迪[1] 廖毅[1] 刘兴楼[1] 李革[1] 舒赛男[1] 方峰[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院儿科病毒实验室,武汉430030

出　　处：《中国免疫学杂志》2018年第1期31-35,共5页Chinese Journal of Immunology

基　　金：国家自然科学基金(81271807)资助项目

摘　　要：目的:在整体水平研究促炎细胞因子IL-17在巨细胞病毒肝炎发病机制中的作用。方法:首先建立MCMV全身播散性感染模型,设立正常对照组、MCMV感染对照组、IL-17阻断组和同型抗体对照组。在实验第7天处死小鼠,Western blot检测各组肝脏组织中IL-17蛋白表达水平,病理评估各组肝组织损伤程度;罗氏生化分析仪检测小鼠血清ALT水平;双抗体夹心ELISA法检测血清中IL-17水平;RT-PCR检测肝脏组织内IL-17R、IFN-γ和IL-10 mRNA的表达。结果:与MCMV感染对照组及同型抗体对照组相比较,IL-17抗体阻断肝脏组织IL-17表达明显下降(P<0.05),病理损伤程度明显减轻,ALT水平显著降低[(146±15)vs(102±11)vs(37±12),P<0.05];血清中IL-17水平降低[(719.76±6.06)vs(722.1±4.62)vs(707.53±8.58),P<0.05];IFN-γ[(0.56±0.06)vs(0.55±0.13)vs(0.96±0.2),P<0.05]与IL-10 mRNA[(0.55±0.073)vs(0.51±0.07)vs(0.903±0.18),P<0.05]的表达明显增加,而IL-17R mRNA表达差异无显著统计学意义[(0.81±0.16)vs(0.89±0.38)vs(0.87±0.23),P>0.05]。结论:促炎因子IL-17高表达参与了巨细胞病毒肝炎的免疫损伤过程,阻断IL-17有助于改善肝功能,减轻肝组织病理损伤。Objective： To investigate the role of pro-inflammatory cytokine IL-17 involved in the pathogenesis of cytomegalovirus hepatitis in vivo. Methods： First of all,disseminated infection model was established. Then,mice were randomly divided into 4 groups： normal control group,MCMV-infected control group,IL-17 blockade group,and isotype control group. Mice were sacrificed on day 7 after infection. The levels of IL-17 protein were detected by Western blot. Hematoxylin eosin（ HE） staining was performed to evaluate the pathologic change of the liver. Serum ALT levels were detected by a Roche DPPI biochemical analyzer. The level of serum IL-17 was measured by double antibody sandwich ELISA. The expressions of mRNA of IL-17 R,IFN-γ and IL-10 in liver were detected by RT-PCR. Results： Compared with MCMV-infected mice and isotype control,the blockade of IL-17 inhibited the expression of IL-17 protein in liver（ P〈0. 05）. The degree of liver damage reduced obviously. The serum ALT was significantly lower[（ 146±15）vs（ 102±11） vs（ 37±12）,P〈0. 05]. The level of serum IL-17 was relatively reduced[（ 719. 76±6. 06） vs（ 722. 1±4. 62） vs（ 707. 53±8. 58）,P〈0. 05]. The expression of IFN-γ mRNA [（ 0. 56 ± 0. 06） vs（ 0. 55 ± 0. 13） vs（ 0. 96 ± 0. 2）,P〈0. 05] and IL-10 mRNA[（ 0. 55±0. 073） vs（ 0. 51±0. 07） vs（ 0. 903±0. 18）,P〈0. 05]increased significantly,while that of IL-17 R did not change apparently[（ 0. 81±0. 16） vs（ 0. 89±0. 38） vs（ 0. 87±0. 23）,P〈0. 05]. Conclusion： The increased expression of pro-inflammatory cytokine IL-17 is involved in the pathogenesis of immune injury in cytomegalovirus hepatitis. The blockade of IL-17 is helpful to relieve the liver damage and improve the liver function.

关 键 词：IL-17 巨细胞病毒肝炎 免疫病理性损伤 

分 类 号：R392[医药卫生—免疫学]