立方液晶纳米粒在MDCK-MDR1细胞模型中的摄取及摄取机制研究  

Study on the cellular uptake and the up-take mechanism of cubic liquid crystalline nanoparticle on MDCK-MDR1 cells model

作　　者：谭宁[1] 杜守颖[1] 薛宇涛 谭丽[1] 陆洋[1] 李鹏跃[1] 

机构地区：[1]北京中医药大学中药学院,中药药剂学研究室,北京100102

出　　处：《天津中医药》2018年第1期56-59,共4页Tianjin Journal of Traditional Chinese Medicine

基　　金：北京市自然科学基金项目(7164274);2017年北京中医药大学研究生自主课题项目(2017-JYB-XS-072)

摘　　要：[目的]考察MDCK-MDR1细胞对立方液晶纳米粒的摄取及摄取机制。[方法]以钙黄绿素为标准荧光物质制备液晶纳米粒。采用流式细胞仪检测不同时间点MDCK-MDR1细胞内荧光强度,比较细胞对钙黄绿素、钙黄绿素液晶纳米粒摄取的差异;采用不同抑制剂(非律平、细胞松弛素D、氯丙嗪、2-D-去氧葡萄糖)与钙黄绿素液晶纳米粒共同孵育后,流式细胞仪测定胞内荧光强度,判断MDCK-MDR1细胞摄取液晶纳米粒的通路。[结果]摄取2 h内,立方液晶纳米粒不仅可以增加MDCK-MDR1细胞对钙黄绿素的摄取也可改变细胞的摄取行为。经氯丙嗪、2-D-去氧葡萄糖孵育的细胞胞内荧光含量低,差异有统计学意义(P<0.05)。[结论]立方液晶纳米粒可增加MDCK-MDR1对钙黄绿素的摄取,摄取途径为能量依赖网格蛋白介导的主动内吞。[Objective] To study cellular uptake and pathway of cubic liquid crystalline nanoparticles（LCNPs） on MDCK-MDR1 cells.[Methods] Make LCNPs with calein（Cal-LCNPs）,the standard fluorescent substance.Fluorescence intensity in MDCK-MDR1 cells was measured by flow cytometry at different time points.Different inhibitors（Filipin,Phytoalexins D,2-Deoxy-D-glucose,chlorpromazine）were incubated with Cal-LCNPs.Flow cytometry was used to determine the intracellular fluorescence intensity then determine the pathways.[Results] Cubic liquid crystal nanoparticles can not only increase the uptake of Cal in MDCK-MDR1 cells in 2 h,but also alter the uptake behavior of cells.MDCK-MDR1 uptakes Cal was consistent with the zero-order equation,and Cal-LCNPs＇ was in accordance with the first-order equation.The intracellular fluorescence of cells incubated with chlorpromazine and 2-D-deoxyglucose was low conpared with control（P0.05）.[Conclusion] Cubic liquid crystal nanoparticles can increase the uptake of calcein by MDCK-MDR1,and the pathway is energy-dependent clathrin mediated endocytosis initiative.

关 键 词：MDCK-MDR1 立方液晶纳米粒 摄取 机制 通路抑制剂 

分 类 号：R94[医药卫生—药剂学]