鞘内注射小豆蔻明对大鼠糖尿病神经痛的影响  

Effect of intrathecal cardamonin on diabetic neuropathie pain in rats

作　　者：何万友[1] 叶丽[1] 任杰平[1] 贺俭[1] 熊青明 王汉兵[1] He Wanyou;Ye Li;Ren Jieping;He Jian;Xiong Qingming;Wang Hanbing(China Department ofAnesthesiology, First People＇s Hospital of Foshan, Foshan 528000,Chin)

机构地区：[1]佛山市第一人民医院麻醉科,528000

出　　处：《中华麻醉学杂志》2017年第12期1469-1472,共4页Chinese Journal of Anesthesiology

基　　金：广东省自然科学基金（2014A030313719）

摘　　要：目的评价鞘内注射小豆蔻明对大鼠糖尿病神经痛（DNP）的影响。方法健康成年雄性SD大鼠，2月龄，体重180～220 g，鞘内置管后腹腔注射链脲佐菌素60 mg/kg制备大鼠糖尿病模型。造模后21 d痛阈下降〉50%基础值为DNP造模成功。取DNP大鼠24只，采用随机数字表法分成4组（n＝6）：DNP组、雷帕霉素组（R组）、小豆蔻明50 μg组（D50组）和小豆蔻明100 μg组（D100组），另取健康同龄大鼠6只作为对照组（C组）。DNP组、R组、D50组和D100组于糖尿病造模后21 d分别鞘内注射二甲基亚砜10 μl、雷帕霉素5 μg、小豆蔻明50 μg和小豆蔻明100 μg，1次/d，连续7 d。于糖尿病造模前、造模后7、14、21 d、鞘内给药后1、4、7 d时测定MWT。最后一次测定MWT后取L3-5脊髓，采用Western blot法测定S6K、p-S6K和突触素Ⅱ的表达。结果与C组比较，DNP组MWT降低，脊髓S6K、p-S6K和突触素Ⅱ表达上调（P〈0.05或0.01）；与DNP组比较，R组、D50组和D100组MWT升高，脊髓S6K、p-S6K和突触素Ⅱ表达下调（P〈0.05或0.01）；与D50组比较，D100组MWT升高，脊髓S6K、p-S6K和突触素Ⅱ表达下调（P〈0.05）。结论鞘内注射小豆蔻明可缓解大鼠DNP，机制与抑制脊髓雷帕霉素靶蛋白活性，下调突触素Ⅱ表达有关。Objective To evaluate the effect of intrathecal cardamonin on diabetic neuropathic pain（DNP）in rats.Methods Healthy adult male Sprague-Dawley rats, aged 2 months, weighing 180-220 g, were included in the study.Diabetes mellitus was produced by intraperitoneal injection of streptozotocin 60 mg/kg after intrathecal catheterization.When decrease in pain threshold〉50% of the baseline at 21 days after diabetes mellitus was produced, the induction of DNP was considered successful.Twenty-four rats with DNP were divided into 4 groups（n=6 each）using a random number table： group DNP, rapamycin group（group R）, cardamonin 50 μg group（group D50）and cardamonin 100 μg group（group D100）. Another 6 healthy age-matched rats were used as normal control（group C）. In DNP, R, D50 and D100 groups, dimethyl sulfoxide 10 μl, rapamycin 5 μg and cardamonin 50 μg and 100 μg were intrathecally injected, respectively, once a day for 7 consecutive days starting from 21 days after successful establishment of the model.Mechanical paw withdrawal threshold（MWT）was measured before establishment of the model, on 7, 14 and 21 days after establishment of the model, and on 1, 4 and 7 days after intrathecal injection.The rats were sacrificed after the last MWT measurement, and their L3-5 segments of the spinal cord were removed for determination of the expression of S6K, p-S6K and synapsin Ⅱ by Western blot.Results Compared with group C, MWT was significantly decreased, and the expression of spinal S6K, p-S6K and synapsin Ⅱ was up-regulated in group DNP（P〈0.05 or 0.01）. Compared with group DNP, MWT was significantly increased, and the expression of spinal S6K, p-S6K and synapsin Ⅱ was down-regulated in R, D50 and D100 groups（P〈0.05 or 0.01）. Compared with group D50, MWT was significantly increased, and the expression of spinal S6K, p-S6K and synapsin Ⅱ was down-regulated in group D100（P〈0.05）.Conclusion Intrathecal cardamonin can relieve DNP in rats, and the mechanism is related to inhibiti

关 键 词：姜科 注射 脊髓 糖尿病 神经痛 

分 类 号：R587.2[医药卫生—内分泌] R747.9[医药卫生—内科学]