脊髓电刺激对糖尿病神经痛大鼠脊髓神经型一氧化氮合酶表达的影响  

Effect of spinal cord stimulation on expression of neuronal nitric oxide synthase in rats with diabetic neuropathic pain

作　　者：黄翔[1] 袁雅婷 李晓宏[1] 李世杰[1] 邓翠芬[1] Huang Xiang;Yuan Yating;Li Xiaohong;Li Sh;ijie;Deng Cuifen(Department of Pain Management, First People＇s Hospital of Foshan, Foshan 528000, Chin)

机构地区：[1]佛山市第一人民医院疼痛科,528000

出　　处：《中华麻醉学杂志》2017年第12期1482-1484,共3页Chinese Journal of Anesthesiology

摘　　要：目的评价脊髓电刺激对糖尿病神经痛大鼠脊髓神经型一氧化氮合酶（nNOS）表达的影响。方法清洁级健康雄性SD大鼠48只，2月龄，体重250～300 g，采用随机数字表法分为4组（n＝12）：对照组（C组）、糖尿病组（DM组）、假手术组（S组）和脊髓电刺激组（SCS组）。采用腹腔注射1%链脲佐菌素（STZ）的方法制备大鼠糖尿病模型，注射STZ后2 d血糖〉16.7 mmol/L为模型制备成功。S组和SCS组于造模成功后19 d在硬膜外间隙置入电极，SCS组于造模成功后26～28 d时行脊髓电刺激（频率50 Hz，脉冲宽度0.2 ms，电流强度为引起后肢轻微抽动的最低强度的2/3）30 min，1次/d。于造模前1 d（T1）、造模成功后2、7、14和28 d（T2-5）时测定机械缩足反应阈（MWT）。于T5时测定痛阈后处死大鼠，取腰段脊髓，检测NO含量及nNOS表达。结果与C组比较，DM组、S组和SCS组T4，5时MWT降低，脊髓NO含量和nNOS表达水平升高（P〈0.05）；与DM组比较，SCS组T5时MWT升高，脊髓NO含量和nNOS表达水平降低（P〈0.05）。结论脊髓电刺激减轻大鼠糖尿病神经痛的机制与抑制nNOS表达上调，减少NO生成有关。Objective To evaluate the effect of spinal cord stimulation on the expression of neuronal nitric oxide synthase（nNOS）in the rats with diabetic neuropathic pain.Methods Forty-eight pathogen-free healthy male Sprague-Dawley rats, aged 2 months, weighing 250-300 g, were divided into 4 groups（n=12 each）using a random number table： control group（group C）, diabetes mellitus group（group DM）, sham operation group（group S）and spinal cord stimulation group（group SCS）. Diabetic neuropathic pain was produced by intraperitoneal injection of 1% streptozocin 75 mg/kg and confirmed by blood glucose level〉16.7 mmol/L on day 2 after streptozocin injection.Electrodes were implanted into the epidural space at 19 days after successful establishment of the model in S and SCS groups, and in addition spinal cord stimulation（frequency 50 Hz, pulse width 0.2 ms, intensity=2/3 of the lowest intensity）was performed for 30 min once a day at 26-28 days after successful establishment of the model in group SCS.The mechanical paw withdrawal threshold（MWT）was measured at 1 day before establishment of the model（T1）and 2, 7, 14 and 28 days after successful establishment of the model（T2-5）. The rats were sacrificed after the last measurement of MWT at T5, and the lumbar segment of the spinal cord was removed for determination of nitric oxide（NO）content and nNOS expression in spinal cord tissues.Results Compared with group C, the MWT was significantly decreased at T4, 5, and the NO content and nNOS expression in the spinal cord were increased in DM, S and SCS groups（P〈0.05）. Compared with group DM, the MWT was significantly increased at T5, and the NO content and nNOS expression in the spinal cord were decreased in group SCS（P〈0.05）.Conclusion The mechanism by which spinal cord stimulation attenuates diabetic neuropathic pain is related to inhibiting nNOS expression and reducing NO production in rats.

关 键 词：脊髓 电刺激疗法 糖尿病 神经痛 一氧化氮合酶 

分 类 号：R587.2[医药卫生—内分泌] R747.9[医药卫生—内科学]