长江中下游湖泊短颌鲚线粒体控制区遗传多样性分析  

Genetic Diversity of Coilia brachygnathus in Yangtze River Based on Mitochondrial DNA D-loop Region Sequences

作　　者：王旭歌[1,2] 何勇凤[1] 吴兴兵[1] 杨德国[1] 

机构地区：[1]中国水产科学研究院长江水产研究所,农业部淡水生物多样性保护重点实验室,武汉430223 [2]上海海洋大学,上海201306

出　　处：《动物学杂志》2018年第1期106-113,共8页Chinese Journal of Zoology

基　　金：现代农业产业技术体系专项资金(No.CARS-46); “十二五”国家科技支撑计划课题(No.2012BAD25B08-03)

摘　　要：为探明长江中下游不同湖泊中短颌鲚(Coilia brachygnathus)遗传多样性水平和遗传分化程度,以洞庭湖、长湖、巢湖3个地理群体作为研究对象,采用线粒体控制区序列为分子标记,分别应用软件Dna SP 5.0、Arlequin3.1.1、MEGA5.0和Network 5.1进行了遗传参数统计和单倍型间分子变异分析(AMOVA),构建邻接系统树及单倍型网络图。对长江中下游短颌鲚野生群体的遗传多样性和遗传结构进行分析。结果显示,用来分析的1 236 bp D-loop区序列中共90个变异位点,54个简约信息位点。长江中下游3个地理群体中共发现58个单倍型,单倍型多样性(h)范围0.949~0.982,核苷酸多样性范围0.004 99~0.006 21,说明长江中下游3个湖泊短颌鲚地理群体具有较高的遗传多样性水平。3个短颌鲚地理群体遗传分化指数(Fst)为0.265 95,呈现出中等程度的分化水平,主要表现在巢湖群体与其他群体之间处于中等程度分化水平。依据遗传距离构建系统发育树及单倍型网络图也出现相类似结果。To assess the genetic diversity and genetic variation of Coilia brachygnathus populations in the mid-lower Yangtze River, three geographical populations from Dongting Lake, Changhu and Chaohu were studied based on mitochondrial D-loop sequences. Some genetic diversity parameters were analyzed by Dna SP 5.0, and heterozygous molecular variation analysis（AMOVA） was acquired by Arlequin 3.1.1. Using MEGA5.0, we analyzed the base composition, the number of nucleic acid site replacement, the calculation ofthe genetic distance between populations and among populations. We also constructed the neighbor joining（NJ） phylogenetic tree, and the NJ system tree based on the topology of each branch. Network 5.1 was used to analyze the sequence of the control zone, and then the haplotype network diagram was constructed. Genetic diversity and genetic structure of C. brachygnathus populations in the mid-lower Yangtze River were analyzed. The results showed that there were 1 236 bp, 90 variation sites and 54 parsimony informative sites in D-loop sequence. There were 58 haplotypes among three geographical populations in the mid-lower Yangtze River, and the haplotypes diversity range was 0.949﹣0.982, the diversity of nucleotides was in the range of 0.004 99﹣0.006 21, indicating that the three geographical groups in the mid-lower Yangtze River had a high genetic diversity level（Table 2, 3）. The genetic differential index Fst of total for populations was 0.265 95（Table 4）, showing that the genetic differentiation among populations was moderate and significant, especially between the Chaohu population and other groups. Similar results were also obtained according to the NJ phylogenetic tree and haplotype network diagram（Fig. 1, 2）.

关 键 词：短颌鲚 D-LOOP 遗传多样性 遗传分化 地理群体 

分 类 号：Q953[生物学—动物学]