禽白血病病毒K亚群RT-LAMP检测方法的建立  

Development of RT-LAMP method for the detection of avian leukosis virus subgroup K

作　　者：黄海超 陈轩 杨素 赵海燕 曹伟胜[2] 邵建宏 赵福振 沙才华 李忠 

机构地区：[1]珠海出入境检验检疫局检验检疫技术中心,广东珠海519000 [2]华南农业大学兽医学院,广东广州510642

出　　处：《中国兽医科学》2018年第2期161-166,共6页Chinese Veterinary Science

基　　金：国家自然科学基金项目(31672552);广东省自然科学基金项目(2016A030313403);广东省家禽产业技术体系疾病控制岗位专家项目(2016LM1114);国家肉鸡产业技术体系项目(CARS-41-G16);2015年国家质量监督检验检疫总局科技计划项目(2015IK337);珠海出入境检验检疫局科技项目(ZH2016-6)

摘　　要：根据Gen Bank数据库中禽白血病病毒（ALV）K亚群特异性的gp85基因序列,设计了特异性检测ALV K亚群（ALV-K）的RT-LAMP引物。对内、外引物浓度组合和反应温度进行优化,建立了ALV-K的RT-LAMP检测方法。特异性试验结果显示,本方法只对ALV-K进行特异性扩增,而对禽白血病的其他亚群以及NDV、REV、AIV、MDV、IBV等常见禽病病原未见扩增。敏感性试验表明,本方法最低能够检测到3.4×104copies/L的质粒。Based on the gp85 gene sequences of avian leukosis virus subgroup K fromGenBank database, the specific RT-LAMPprimers were designed to detect avian leukosis virus subgroup K （ALV-K）. The dif- ferent concentration combinations of inner and outer primers were tested and reaction temperature was optimized to establish the RT-LAMPmethod for detecting ALV-K. The results showed that this method can specifically detect ALV-K without cross-reactions with other subgroups of ALV or other poultry disease pathogens such as NDV,REV,AIV,MDV,IBV,etc..The sensitivity test indicated that the minimum detectable limit was 3.4× 104copies of plasmids.

关 键 词：禽白血病病毒 K亚群 RT-LAMP 检测方法 

分 类 号：S852.659.3[农业科学—基础兽医学]