旋毛虫cystatin-like基因重组蛋白诊断特性的鉴定  

Diagnostic identification of cystatin-like gene recombinant protein in Trichinella spiralis

作　　者：翟铖铖 陈家旭[1] 陈韶红[1] 艾琳[1] 卢艳[1] 蔡玉春[1] 俞英昉[1] 田利光[1] 吴秀萍[1] 

机构地区：[1]中国疾病预防控制中心寄生虫病预防控制所世界卫生组织热带病合作中心科技部国家级热带病国际联合研究中心卫生部寄生虫病原与媒介生物学重点实验室

出　　处：《中国兽医科学》2018年第2期189-195,共7页Chinese Veterinary Science

基　　金：国家重点研发计划项目(2017YFD0501303)

摘　　要：为鉴定旋毛虫肠道期肌幼虫cystatin-like基因重组蛋白的诊断特性,利用ELISA方法,以旋毛虫cystatin-like基因重组蛋白作为包被抗原,并以旋毛虫感染早期肠道6 h幼虫ES抗原作为阳性对照,用不同旋毛虫感染剂量、不同感染时间小鼠血清为一抗,检测血清中抗旋毛虫Ig G抗体的变化情况,利用Excel软件绘制出Ig G抗体消长规律曲线并进行数据分析,鉴定该基因重组蛋白作为诊断抗原时的敏感性及诊断效果。结果显示,在低剂量感染和高剂量感染时,该基因重组抗原分别从感染后第10天和第8天起可以稳定地检测出抗旋毛虫Ig G抗体;在感染后第11天至第45天一直呈“波峰波谷”式并维持较高水平;感染早期（第15~45天）低剂量感染组的Ig G抗体水平明显高于高剂量组。由此推断,旋毛虫cystatin-like基因重组蛋白具有很好的早期诊断特性,尤其对低剂量早期感染的诊断效果更佳,并推测该基因极有可能在旋毛虫感染早期对宿主的免疫抑制作用中起重要作用,其具体应用前景和生物学功能还有待于进一步研究。To identify the diagnostic characteristics of cystatin-like gene protein of the in- testinal stage larvae of Trichinella spiralis,ELISA was used using the cystatin-like gene recombinant protein as envelope antigen and the excretory-secretory （ES） antigen of early intestinal 6 hours muscle larvae （6hML） was made as positive control to detect the level of anti-Trichinella antibody IgG with different infection doses and days post infection （dpi）.The antibody IgG rules were mapped by Microsoft Excel,which indicated the sensitivity and the effects for diagnosis of cystatin-like gene recombinant protein. The results showed that,in all groups,the anti-Trichinella antibody IgG was positive steadily from the i0 dpi and 8 dpi;the curve of antibody IgG was ＂crest wave trough＇type and maintained at a high level from 11 dpi to 45 dpi;antibody IgG level was significantly higher at the early stage （from 15 dpi to 45dpi） in low dose group. Therefore,cystatin-like gene recombinant protein has excellent diagnostic ef- fects for early serodiagnosis,especially in low dose infection,and it is speculated that cystatin-like gene is likely to play an important role in host immune inhibition at a early stage of Trichinella infection. Biology functions of cystatin-like gene recombinant protein remain to be further studied.

关 键 词：旋毛虫 cystatin-like基因 基因重组蛋白 早期诊断 

分 类 号：S852.731[农业科学—基础兽医学]