α-硫辛酸对亚慢性镉暴露致大鼠大脑皮质超微结构损伤的保护效应  

Protective effects of α-lipoic acid on ultrastructure damage by sub-chronic cadmium exposure in cerebral cortex of rats

作　　者：赵世文[1,2] 杨金龙 陈洁[1,2] 王涛 邹辉 顾建红[1,2] 刘学忠 卞建春[1,2] 刘宗平 袁燕[1,2] 

机构地区：[1]扬州大学兽医学院,江苏扬州225009 [2]江苏高校动物重要疫病与人兽共患病防控协同创新中心,江苏扬州225009

出　　处：《中国兽医科学》2018年第2期256-261,共6页Chinese Veterinary Science

基　　金：国家重点研发计划项目(2016YFD0501208);江苏高校优势学科建设工程资助项目(PAPD);国家自然科学基金资助项目(31772808);中国博士后科学基金资助项目(2015M581874);江苏省博士后科研资助计划项目(1501072A)

摘　　要：为探讨α-硫辛酸（α-LA）在亚慢性镉暴露引起大鼠大脑皮质超微结构损伤中的保护作用,24只21日龄雌性SD大鼠被随机分为4组,分别为对照组、α-LA组、镉组和镉与α-LA共处理组,镉组采用50 mg/L醋酸镉溶液以自由饮水方式进行染毒,共处理组在镉暴露的基础上同时用α-LA溶液按50 mg/（kg·d）进行灌胃。12周后用原子吸收分光光度法测定大鼠大脑皮质中镉的质量分数,透射电镜观察大脑皮质组织超微结构的变化,Western-blot检测凋亡相关蛋白的表达情况。结果表明,α-LA能够显著降低镉引起的大鼠大脑皮质镉质量分数的升高（P〈0.05）,缓解镉引起的超微结构损伤,抑制镉导致的Bcl-2/Bax比值下降和Cleaved caspase-9,-3表达量的升高（P〈0.05或P〈0.01）。这说明α-LA对亚慢性镉暴露致大鼠大脑皮质超微结构的损伤具有保护作用。In order to investigate the protective effects of α-lipoic acid（α-LA） on ultrastructure damage by sub chronic cadmium（Cd） exposure in cerebral cortex of rats,twenty-four 21-day old female SD rats were divided into 4 groups randomly：control group,α-LA group,Cd-treated group,Cd and α-LA co-treated group. The rats of Cd-treated group were exposed to Cd（50 mg/L） in drinking water,and α-LA（50 mg/kg） was given to rats by gavage at the same time in the co-treated group. After 12 weeks,the mass con- centration of Cd in cerebral cortex was measured by atomic absorption spectrophotometer,the changes in ultrastructure of the cerebral cortex were observed under transmission electron microscope and the expression of apoptosis related protein was measured by Western-blot. The results showed that compared with Cd group,α-LA could decrease the mass concentration of Cd in cerebral cortex significantly（P〈0. 05）,relieve the ultrastructural damage,and inhibit the decline of Bcl-2/Bax ratio and the increase of protein expression of cleaved caspase-9 and -3 caused by Cd significantly（P〈0. 05 or P〈0.01）.The results indicated that α-LA could protect the ultrastructure damage by sub-chronic cadmium exposure in cerebral cortex of rats.

关 键 词：亚慢性 镉 Α-硫辛酸 SD大鼠 超微结构 大脑皮质 

分 类 号：S854.53[农业科学—临床兽医学]