双显色体系计算分光光度法测定银杏单宁含量  

Determination of Ginkgo Biloba Tannin Content by Dual Chromophoric Systems Computational Spectrophotometry

作　　者：张丝柳 郭丽萍 杜小弟 胡婧 雷家珩 

机构地区：[1]武汉理工大学化学化工与生命科学学院

出　　处：《化学试剂》2018年第2期164-166,170,共4页Chemical Reagents

摘　　要：为了消除分光光度法测定单宁过程中黄酮类物质产生的干扰,采用酒石酸亚铁和硝酸铝-亚硝酸钠双显色体系进行测定,并通过计算分光光度法求解二元方程组获得单宁含量,同时也解得黄酮含量。单宁和黄酮分别在酒石酸亚铁显色体系和硝酸铝-亚硝酸钠显色体系中都具有线性响应,线性相关系数在0.998 7～0.999 9范围内。酒石酸亚铁体系中,ε_（单宁1）=0.010 32 L/（mg·cm）,ε_（黄酮1）=0.005 750 L/（mg·cm）;硝酸铝体系中,ε黄酮2=0.012 45 L/（mg·cm）,ε_（单宁2）=0.002707 L/（mg·cm）。在实际混合体系中,吸光度符合加和性,通过方程组解得的单宁含量标准偏差为0.024%～0.062%,加标回收率为101.8%～108.0%,说明本方法精密度和准确度较高。同时还求得黄酮含量标准偏差为0.013%～0.033%,加标回收率为102.7%～104.9%。通过此方法测出银杏叶（春）、银杏叶（秋）、外种皮、白果中的单宁含量分别为1.605%、1.031%、0.947%、0.055%。To eliminate the interference of flavonoids in the determination of tannins by spectrophotometry,this work adopted the ferrous tartrate and aluminum nitrate-sodium nitrite dual chromophoric systems,and tannin content was calculated by computational spectrophotometry,flavonoid content was obtained as well.There were linear relationships both of tannins and flavonoids in ferrous tartrate chromogenic system and aluminum nitrate-sodium nitrite chromogenic system,and the linear correlation coefficient in the range of 0.998 7 to 0.999 9.In the ferrous tartrate system,ε_（tannin1）= 0.010 32 L/（mg·cm）,ε_（flavonoid1）= 0.005 750 L/（mg·cm）;In aluminum nitrate-sodium nitrite system,ε_（flavonoid2）= 0.012 45 L/（mg·cm）,ε_（tannin2）= 0.002 707 L/（mg·cm）.In the mixed system,the absorbance conformed to the additive property,through the equations,the standard deviation of tannin content was0.024% ~ 0.062%,the recovery was range from 101.8% to 108.0%,it means that this method has high precision and accuracy.At the same time,the standard deviation of flavonoid content was determined which was 0.013% to 0.033%,and the recovery was range from 102.7% to 104.9%.Via this method,the tannin content of ginkgo leaves（spring）,ginkgo leaves（autumn）,ginkgo sarcotestas,ginkgo seeds were 1.605%,1.031%,0.947% and 0.055%,respectively.

关 键 词：银杏 单宁 计算分光光度法 酒石酸亚铁光度法 硝酸铝-亚硝酸钠光度法 

分 类 号：O657.32[理学—分析化学]