脑络欣通胶囊对脑缺血再灌注损伤大鼠血小板膜蛋白的影响  

Effect of Naoluo Xintong capsules on membranous glucoprotein in rats after focal cerebral ischemia/reperfusion injury

作　　者：赵丽[1,2,3] 汪宁 朴新新[1,2,3] 杨飞雪 王键 

机构地区：[1]新安医学教育部重点实验室,合肥230012 [2]安徽省中医药科学院中药药效与安全性评价研究所,合肥230012 [3]安徽中医药大学,合肥230012

出　　处：《中南药学》2018年第1期40-44,共5页Central South Pharmacy

基　　金：国家科技支撑计划(No.2012BAI26B03);安徽省高校学科(专业)拔尖人才学术资助目(No.gxbjZD15)

摘　　要：目的研究脑络欣通胶囊对局灶性脑缺血再灌注损伤大鼠血小板膜蛋白的影响。方法雄性健康SD大鼠随机分为假手术组10只,造模组70只,采用改良线栓法制备大鼠局灶性脑缺血再灌注损伤模型,选取造模成功的大鼠随机分为模型组,阿司匹林组,血府逐瘀颗粒组,脑络欣通高、中、低剂量组(4.50、2.25、1.13 g·kg-1)。采用ELISA法测定血浆中环磷酸腺苷(cyclic adenosine monophosph,c AMP)、血管性假血友病因子(von Willebrand factor,v WF)的含量;流式细胞仪检测GPⅡb/Ⅲa复合体(CD41/CD61)、血小板颗粒膜蛋白140(GMP140,CD62P)。结果与模型组比较,脑络欣通高、中剂量组v WF均降低(P<0.05)、c AMP升高(P<0.05,P<0.01),血小板膜糖蛋白CD41/CD61及CD62P活性降低(P<0.01)。结论脑络欣通胶囊可抑制局灶性缺血再灌注后血小板膜糖蛋白的表达,对缺血性脑血管疾病具有一定的保护作用。Objective To determine the effect ofNaoluo Xintong capsules on membranous glucoprotein in rats with focal cerebral ischemia/reperfusion injury. Methods Healthy male SD rats were grouped into a sham operation group （n = 10） and a model group （n = 70） randomly. Middle cerebral artery occlusion/reperfusion （MCAO/ R） models were established by the improved intraluminal suture method. The successfully modeled rats were randomly divided into a model group, an Aspirin group （0.01 g kg-1）, a Xuefu Zhuyu granule group, and Naoluo Xintong high, middle and low dose groups （4.50, 2.25, and 1.13 g kg-1）. The cAMP and vWF were measured by enzyme linked immunosorbent assay for 2 h followed by 24 h reperfusion, and the GP Ⅱ b/Ⅲ a complex （CD41/ CD61） and platelet granule membrane protein 140 （GMP140, CD62P） were detected by flow cytometry. Results Compared with that of the model group, the vWF content in the plasma of rat model was reduced （P 〈 0.05）, and the content of cAMP in the plasma was increased （P 〈 0.05, P 〈 0.01） in the high and middle dose groups. In addition, Naoluo Xintong capsules can reduce the positive expression rate of platelet membranous glycoprotein CD41/CD61 and mean fluorescence intensity of CD62P （P 〈 0.01）. Conclusion Naoluo Xintong capsules can effectively inhibit the expression of membranous glycoproteins in rats with cerebral ischemia-reperfusion injury, with certain protective effect on ischemic cerebral vascular diseases.

关 键 词：脑络欣通胶囊 脑缺血再灌注损伤 大鼠中动脉阻塞 血小板膜糖蛋白 

分 类 号：R285.5[医药卫生—中药学]