左西孟旦对猪冠状动脉微栓塞后心肌细胞凋亡的影响  

Effect of levosimendan on cardiomyocyte apoptosis after coronary microembolization in swine

作　　者：王江友[1] 陈涵[1] 鄢华[1] 宋丹[1] 刘成伟[1] 彭剑[1] 吴明祥[1] 苏晞[1] 

机构地区：[1]武汉亚洲心脏病医院心内科,430022

出　　处：《中华老年心脑血管病杂志》2018年第1期78-82,共5页Chinese Journal of Geriatric Heart,Brain and Vessel Diseases

基　　金：武汉市卫生计生科研基金(WX17Q36)

摘　　要：目的探讨左西孟旦对猪急性冠状动脉微栓塞(coronary microembolization,CME)后心肌细胞凋亡的影响及意义。方法健康巴马系小型猪15只,随机分为假手术组,CME组及干预组[0.05μg/(kg·min)左西孟旦预处理24h],每组5只。成功建立CME术后12h采用心脏超声检测心功能,TUNEL染色检测心肌细胞凋亡,Western blot检测活化半胱氨酸天冬氨酸蛋白酶3(Caspase-3)表达水平。结果与假手术组比较,CME组LVEF[(49.82±4.23)%vs(66.72±3.22)%]、左心室短轴缩短率及心排血量下降,左心室舒张末内径增加[(39.26±1.28)mmvs(32.38±1.58)mm,P<0.05];与CME组比较,干预组心功能明显改善(P<0.05)。与假手术组比较,CME组心肌细胞凋亡率[(10.558±2.425)%vs(0.242±0.070)%]、活化Caspase-3表达显著增加(P<0.05);与CME组比较,干预组心肌细胞凋亡率[(6.820±1.974)%vs(10.558±2.425)%]、活化Caspase-3表达明显减少,差异有统计学意义(P<0.05)。结论左西孟旦预处理可明显减少CME后心肌细胞凋亡,改善心功能。其机制可能是抑制心肌细胞促凋亡蛋白Caspase-3表达来实现的。Objective To study the effect of levosimendan on cardiomyocyte apoptosis after coronary microembolization（CME）in swine.Methods Fifteen healthy swines were randomly divided into sham operation group,CME group and levosimendan treatment group（5 in each group）.Their cardiac function was assessed by echocardiography,their cardiomyocyte apoptosis was assyed with TUNEL staining,and Caspase-3 expression was detected by Western blot at 12 hafter operation.Results The LVEF was lower,the left ventricular minor axis was shorter and the cardiac output volume was smaller while the LVEDD was longer in CME group than sham operation group（P〈0.05）.The cardiac function was significantly better in CME group than in sham operation group（P〈0.05）.The cardiomyocyte apoptosis rate and Caspase-3 expression level were significantly higher in CME group than in sham operation group（P〈0.05）.The cardiomyocyte apoptosis rate was significantly higher while the Caspase-3 expression level was significantly lower in levosimendan treatment group than in CME group（6.820%±1.974%vs 10.558%±2.425%,P〈0.05）.Conclusion Pretreatment with levosimendan can effecively reduce the cardiomyocyte apoptosis and improve the cardiac function after CME by inhibiting the Caspase-3 expression in cardiomyocytes.

关 键 词：急性冠状动脉综合征 肌细胞 心脏 细胞凋亡 栓塞 半胱氨酸天冬氨酸蛋白酶3 左西孟旦 

分 类 号：R541.4[医药卫生—心血管疾病]