小麦与两种病原菌互作的冷冻切片观察效果  

Observation of cryosectioning technique in interaction of wheat and two pathogens

作　　者：姚娟妮 成玉林 韩青梅[1] 张国云 YAO Juanni;CHENG Yulin;HAN Qingmei;ZHANG Guoyun(State Key Laboratory of Crop Stress Biology for Arid Areas;College of Life Science Northwest A＆F University ,Yangling ,Shaanxi 712100 ,China)

机构地区：[1]西北农林科技大学旱区作物逆境生物学国家重点实验室,陕西杨凌712100 [2]西北农林科技大学生命科学学院,陕西杨凌712100

出　　处：《西北农林科技大学学报（自然科学版）》2017年第12期90-94,共5页Journal of Northwest A&F University(Natural Science Edition)

基　　金：中国博士后科学基金项目(2015M580882)

摘　　要：【目的】制作小麦与条形柄锈菌小麦专化型(Puccinia striiformis f.sp.tritici)和禾谷镰刀菌(Fusarium graminearum)互作的冷冻切片,评判基于冷冻切片观察2种病原菌对小麦侵染过程的效果,为将冷冻切片技术应用于植物病原菌侵染检测提供依据。【方法】以水源11和小偃22为试验材料,分别接种条形柄锈菌小麦专化型和禾谷镰刀菌,取样制备冷冻切片,用Olympus BX51多功能显微镜观察2种病原真菌在侵染小麦叶片和穗部过程中的发育结构。【结果】利用冷冻切片观察到条形柄锈菌小麦专化型和禾谷镰刀菌2种病原真菌结构清晰,小麦组织和细胞形态完整,对比度良好。【结论】冷冻切片技术可用于植物病原菌侵染的预检。【Objective】By making frozen section samples of interactions between wheat and Puccinia striiformis f.sp.tritici and Fusarium graminearum,the infection process of the two pathogens on wheat was observed to provide basis for use of the cryosectioning technique in detecting infection of plant pathogens.【Method】Wheat cultivars Suwon 11 and Xiaoyan 22 were inoculated using the two pathogens,and collected to prepare frozen section samples.The fungal structures of the two pathogens developed in wheat leaf and head tissues were observed using multi-functional microscopy（Olympus BX51）.【Result】The cryosectioning technique was suitable for observing structures of P.striiformis f.sp.tritici and F.graminearuminfected wheat tissues easily,and configurations of wheat tissues and cells were kept in a good shape and had good contrast.【Conclusion】The cryosectioning technique can be used to pre-check infection of plant pathogens.

关 键 词：冷冻切片技术 条形柄锈菌小麦专化型 禾谷镰刀菌 组织学 

分 类 号：Q-336[生物学]