定心藤枝叶中化学成分研究  

Chemical constituents from stems and leaves of Mappianthus iodoides

作　　者：蒋芝华[1,2] 冯兴阳 郭微 王伊童 王婷婷 柴玲 刘艳萍[2] 黄泽豪 付艳辉[1,2,3] 

机构地区：[1]福建中医药大学药学院,福建福州350122 [2]海南师范大学热带药用植物化学教育部重点实验室,海南海口571158 [3]广西壮族自治区中医药研究院,广西中药质量标准研究重点实验室,广西南宁530022

出　　处：《中草药》2018年第2期282-287,共6页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(31660097,21662011,21302181);海南省自然科学基金项目(20162024,20162021);教育部创新团队项目(IRT-16R19);广西中药质量标准研究重点实验室开放课题(桂中重开201504)

摘　　要：目的研究定心藤属植物定心藤Mappianthus iodoides枝叶中的化学成分。方法综合运用正相硅胶柱色谱、反相硅胶柱色谱和Sephadex LH-20凝胶柱色谱以及制备型高效液相色谱等方法进行系统分离,根据理化性质及波谱学数据鉴定化合物的化学结构。结果从定心藤枝叶的90%乙醇提取物中分离得到了16个化合物,分别鉴定为9-hydroxy-4,6-megastigmadien-3-one(1)、9-hydroxy-4,7-megastigmadien-3-one(2)、blumenol A(3)、9,10-dihydroxy-4,7-megastigmadien-3-one(4)、5,12-epoxy-9-hydroxy-7-megastigmen-3-one(5)、5,12-epoxy-6,9-hydroxy-7-megastigmen-3-one(6)、黑麦草内酯(7)、落叶松树脂醇(8)、异落叶松树脂醇(9)、5′-甲氧基落叶松树脂醇(10)、橄榄脂素(11)、去氢双松柏醇(12)、chushizisin I(13)、3,3-didemethoxyverrucosin(14)、4-epi-larreatricin(15)和glycerol monolinoleate(16)。其中化合物1~7为降碳倍半萜类化合物,化合物8~15为木脂素类化合物,化合物16为脂肪酸类化合物。结论所有化合物均为首次从定心藤属植物中分离得到。Objective To study the chemical constituents of the stems and leaves ofMappianthus iodoides. Methods The chemical constituents of M. iodoides were separated and purified by silica gel, ODS, Sephadex LH-20 gel column chromatographies, and preparative HPLC. Their structures were identified by physicochemical properties, spectroscopic analysis, as well as comparisons with the data reported in literature. Results Sixteen compounds were isolated from the 90% ethanol extract of the stems and leaves of M. iodoides, which were identified as 9-hydroxy-4,6-megastigmadien-3-one （1）, 9-hydroxy-4,7-megastigmadien-3-one （2）, blumenol A （3）, 9,10-dihydroxy-4,7-megastigmadien-3-one （4）, 5,12-epoxy-9-hydroxy-7-megastigmen-3-one （5）, 5,12-epoxy-6,9-hydroxy-7- megastigrnen-3-one （6）, loliolide （7）, lariciresinol （8）, isolariciresinol （9）, 5＇-methoxylariciresinol （10）, olivil （11）, dehydrodiconiferyl alcohol （12）, chushizisin I （13）, 3,3-didemethoxyverrucosin （14）, 4-epi-larreatricin （15）, and glycerol monolinoleate （16）. Conclusion All compounds are isolated from the genus Mappianthus for the first time.

关 键 词：茶茱萸科 定心藤 降碳倍半萜 木脂素 黑麦草内酯 落叶松树脂醇 橄榄脂素 去氢双松柏醇 

分 类 号：R284.1[医药卫生—中药学]