草苁蓉正丁醇萃取物的抗氧化活性及物质基础初步研究  

Preliminary study on anti-oxidant activities and material base of n-butanol extract of Boschniakia rossica

作　　者：张海丰 臧皓 沈鹏 滕坤 阮洪生[2] 

机构地区：[1]通化师范学院医药学院,吉林通化134002 [2]浙江医药高等专科学校中药学院,浙江宁波315100

出　　处：《中草药》2018年第2期382-388,共7页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(31100254);吉林省教育厅项目:基于UPLC-Q-TOF-MS的代谢组学技术研究草苁蓉抗衰老的作用机制(吉教科合字[2014]第399号)

摘　　要：目的考察草苁蓉Boschniakia rossica正丁醇萃取部位的体内外抗氧化活性,并初步确定其抗氧化作用的物质基础。方法以清除1,1-二苯基-2-三硝基苯肼(DPPH)、2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐(ABTS)自由基能力、亚铁还原能力实验(FRAP法)为指标,检测草苁蓉不同极性萃取部位的体外抗氧化活性;以D-半乳糖诱导衰老大鼠为模型,考察草苁蓉正丁醇萃取部位对大鼠肝脏组织中丙二醛(MDA)含量及超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性的影响;采用高效液相色谱-质谱联用技术对草苁蓉正丁醇萃取部位化学成分进行初步分析。结果草苁蓉正丁醇萃取部位对DPPH、ABTS自由基具有良好的清除作用,且在亚铁还原实验的FRAP测定中表现良好;与模型组比较,草苁蓉正丁醇萃取部位各剂量组大鼠肝脏组织中MDA、SOD、CAT指标均有显著改善(P<0.01),表现为MDA水平降低,SOD、CAT活性升高,并回调到正常水平以上,其体内抗氧化作用呈现剂量依赖性。经液质联用分析从草苁蓉正丁醇萃取部位中共鉴定出19种化学成分,其中14个为苯丙素苷类(PPGs)。结论草苁蓉正丁醇萃取部位具有很好的抗氧化活性,其主要有效成分为PPGs。Objective To investigate anti-oxidant activities of the n-butanol extract of Boschniakia rossica （BEBR）, and preliminarily determine the material basis of its antioxidant activities. Methods Antioxidant activities of different polarity extraction of BEBR were performed by using 1,1-dipheny-2-trinitrophenylhydrazine （DPPH）, 2,2-azino-bis（3-ethylbenzothiazoline-6-sulfonic acid）, diammonium salt （ABTS） radical scavenging assays and ferric reducing anti-oxidant power as the index. The aging rats induced by D-galactose were used as models to investigate the effects of different n-butanol extract parts on the content of MDA and the activity of SOD and CAT in liver tissue; The chemical composition of BEBR was preliminarily analyzed by HPLC-DAD-ESI-MS/MS. Results BEBR had good scavenging effect on flee radicals of DPPH and ABTS, and well performation in ferrous reduction experiments of FRAP determination. Compared with the model group, all indexes of MDA, SOD, and CAT in liver tissues of rats from different dose groups with BEBR were significantly improved （P 〈 0.01）, which showed that levels of MDA reduced, the activity of SOD and CAT increased and back to above normal levels. The anti-oxidant activity in vivo showed in a dose-dependent manner. Nineteen chemical constituents were tentatively characterized by HPLC-DAD-ESI-MS/MS, of which 14 were phenylpropanoid glycosides （PPGs）. Conclusion BEBR has good anti-oxidant effects and PPGs are the main active constituents.

关 键 词：草苁蓉 苯丙素苷类 抗氧化 1 1-二苯基-2-三硝基苯肼 2 2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐 D-半乳糖 

分 类 号：R285.5[医药卫生—中药学]