大鼠肠道菌群对三七总皂苷体外降解的研究  

Degradation of total saponins of Panax notoginseng by intestinal flora of rats in vitro

作　　者：唐岚[1] 傅璐璐 沈丽婷 孙伯璇 朱转凤 余小兰 吕慧侠[2] 单伟光[1] 

机构地区：[1]浙江工业大学药学院,浙江杭州310014 [2]中国药科大学药学院,江苏南京210009

出　　处：《中草药》2018年第2期396-399,共4页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金面上项目(81673830)

摘　　要：目的探究雌、雄大鼠肠道菌群对三七总皂苷中3种皂苷(三七皂苷R1、人参皂苷Rg1和人参皂苷Rb1)的体外降解作用。方法将三七总皂苷分别与雌、雄大鼠肠道菌群孵育液在厌氧条件下共同培养24 h,测定不同时间点孵育液中3种皂苷的量。结果雌、雄大鼠肠道菌群对人参皂苷Rb1均有降解作用,雄鼠的降解较雌鼠稍快。雌、雄鼠肠道菌群对三七皂苷R1和人参皂苷Rg1均无明显降解作用。结论在离体条件下,三七总皂苷中人参皂苷Rb1会被肠道菌群降解,而三七皂苷R1和人参皂苷Rg1则较为稳定。Objective To investigate the degradation of three kinds of saponins （notoginseng saponin R1, ginsenoside Rg1, and ginsenoside Rb1） from total saponins ofPanax notoginseng （tPNS） by intestinal flora in male and female rats in vitro. Methods tPNS were incubated for 24 h with male and female rats intestinal flora separately in vitro under anaerobic environment. The content of three kinds of saponins was measured at different treatment time. Results The results showed that the intestinal flora both in male and female rats all had the degradation action against ginsenosides Rb1, in which the degradation of male rats was slightly faster than that of female rats. But the intestinal flora both in male and female rats had no obvious degradation to notoginseng saponin R1 and ginsenoside Rg1. Conclusion Under the condition of in vitro, tPNS can be degraded by rats intestinal flora, of which Rb1 was degraded most. while notoginseng saponin R1 and ginsenoside Rg1 were more stable.

关 键 词：肠道菌群 三七总皂苷 降解 三七皂苷R1 人参皂苷RG1 人参皂苷RB1 

分 类 号：R285.5[医药卫生—中药学]