短柄乌头遗传多样性的AFLP分析  

AFLP analysis of genetic diversity of Aconitum brachypodum

作　　者：杜春华 普春霞[2] 刘小莉[2] 杨礼攀[2] 张爱丽[2] 钱子刚[2] 

机构地区：[1]云南省药物研究所云南白药集团创新研发中心,云南省中药和民族药新药创制企业重点实验室,云南昆明650111 [2]云南中医学院,云南昆明650500

出　　处：《中草药》2018年第2期439-443,共5页Chinese Traditional and Herbal Drugs

摘　　要：目的对濒危药用植物短柄乌头Aconitum brachypodum遗传多样性进行研究。方法选用3对引物对短柄乌头4个自然居群105个样品进行了AFLP分析,利用POPGENE32、MEGA4、NTSYS等生物统计软件进行相关参数计算和聚类分析。结果在短柄乌头物种居群间的遗传多样性水平上,多态位点百分率为80.57%,Nei’s基因多样性指数(He)为0.322 9±0.179 8,Shannon’s信息指数(I)为0.472 0±0.251 7。在其居群内,多态位点百分率为12%,He为0.115 4±0.044,I为0.168 0±0.065 3。Nei’s基因分化系数(Gst)为0.864 2。聚类分析表明,禄劝居群和东川居群聚为一支,丽江居群和会泽居群聚为一支。结论短柄乌头物种居群间的遗传多样性丰富,居群内的多样性偏低。居群间存在显著的遗传分化。AFLP对短柄乌头的遗传多样性分析对其保护提供依据。Objective To analyze the genetic diversity ofAconitum brachypodum, an endangered medicinal plants. Methods Three pair primers were used to evaluate the relevant genetic parameters and clustering pattern for four natural populations containing 105 individuals by using POPGENE32, MEGA4, NTSYS and other biometric softwares. Results For A. brachypodum, the Nei＇s genetic diversity index （He）, Shannon＇s information index （I）, percentage ofpolymorphic loci （PPB） were 0.322 9 ± 0.179 8, 0.472 0 ± 0.251 7, and 80.57%, respectively; and the population level were 0.115 4 ± 0.044, 0.168 0±0.065 3, and 12%, respectively. The genetic differentiation index （Gst） was 0.864 2. The clustering results showed that, Luquan and Dongchuan populations are grouped together, Lijiang and Huize populations are clustered together. Conclusion A.brachypodum possesses the relatively high level of genetic diversity at the species level, however, low within populations. This study should provide an initial insights for A.brachypodum germplasm conservation

关 键 词：短柄乌头 AFLP 遗传多样性 聚类分析 种质资源 

分 类 号：R282.12[医药卫生—中药学]