无滴落过滤板结合超高效液相色谱质谱分析血中5种吩噻嗪类抗精神病药物（英文）  

Non-drip Filtration Plates for High Performance LC-MS/MS Analysis of 5 Phenothiazine-class Antipsychotics in Blood

作　　者：王朝虹[1] 赵蒙[1] 褚建新 蒋文慧 李虹 刘勇[1] 

机构地区：[1]最高人民检察院检察技术信息研究中心,北京100040 [2]浙江省检察院,杭州310012 [3]杭州市检察院,杭州310000 [4]毒品分析及禁毒技术公安部重点实验室,昆明650000

出　　处：《刑事技术》2018年第1期11-16,共6页Forensic Science and Technology

基　　金：Ministry of Public Security’s basic project exclusive for reinforcing policing with science and technology(No.2016 GABJC50)

摘　　要：本文建立了一种简单、灵敏的分析方法用于快速测定全血样品中5种吩噻嗪类抗精神病药物。该方法样品前处理过程仅需用Captiva ND Lipids无滴落过滤板去除磷脂和蛋白。取100μL全血样品至Captiva ND Lipids无滴落96孔过滤板,加入400μL含0.1%甲酸的甲醇溶液洗脱,在20 psi的正压下收集过滤液,供UPLC-MS/MS分析。5种化合物在0.2~20ng/m L浓度范围内均获得良好的线性,相关系数(r2)均高于0.999,最小检测限可达3~10pg/m L,日内精密度低于7.0%,日间精密度低于10%。应用本方法对实际案件涉及以上吩噻嗪类抗精神病的药物进行检测,效果令人满意。A simple and sensitive method was developed and validated for rapid analysis of 5 phenothiazine-class antipsychotic drugs in human blood. Sample preparation is very simple, with the Captiva non-drip fltration plates （Captiva ND Lipids） being used only for lipid and protein depletion. 100μL of human blood containing the target drugs was transferred to Captiva ND Lipids 96-well filtration plates, followed by the addition of 400μL of 0.1% formic acid in methanol. The fltrate was then collected under 20 psi pressure and subsequently received into UPLC-MS/MS analysis. All of the analytes gave linear calibration curves in the range of 0.2~20ng/mL with correlation coefficients （r2） better than 0.999. The LODs were 3-10pg/mL. The RSDs for each analyte were less than 7.0% for intra-day precision and below 10% for the inter-day. The proposed method was applied to the determination of 5 phenothiazine-class antipsychotics in several real forensic cases, leaving with satisfactory results.

关 键 词：吩噻嗪类抗精神病药物 血样 无滴落96孔过滤板 UPLC-MS/MS 

分 类 号：DF795.1[医药卫生—法医学]