利用BSMVAgro/LIC进行小麦条锈菌耐高温相关基因功能分析的互作体系构建  

Development of an interactive system for functional analysis of high-temperature tolerance-related genes of wheat stripe rust pathogen by using BSMV Agro/LIC

作　　者：吴艳琴[1] 王金鑫[1] 李雪[1] 高海峰[2] 王凤涛[1] 冯晶[1] 蔺瑞明[1] 徐世昌[1] 

机构地区：[1]中国农业科学院植物保护研究所,植物病虫害生物学国家重点实验室,北京100193 [2]新疆农业科学院植物保护研究所,乌鲁木齐830091

出　　处：《植物保护》2018年第1期45-51,共7页Plant Protection

基　　金：国家重点基础研究发展计划(“973”计划)(2013CB127700); 国家重点研发计划(2016YFD0100102); 植物病虫害生物学国家重点实验室开放基金(SKLOF201509); 农业部作物种质资源保护与利用专项(2017NWB036-12)

摘　　要：为利用HIGS技术研究条锈菌耐高温胁迫相关基因的功能,需要构建小麦品种与条锈菌及大麦条纹花叶病毒Barley stripe mosaic virus(BSMV)亲和互作体系。本研究选用20个常温下(10℃)高感条锈病的小麦品种和22个耐高温条锈菌菌株,进行高温(21℃)条件下接种亲和性分析,发现11个品种高感条锈病、3个菌株耐高温性稳定且毒性谱较宽。在此基础上,利用含有小麦TaPDS基因片段的重组BSMV侵染上述候选小麦品种,确定‘Local Red'等6个品种与BSMV亲和程度符合试验要求。筛选出小麦品种‘Local Red'与菌株15335-2、15323-4等组成的最佳互作组合,适合利用BSMV Agro/LIC方法开展小麦条锈菌耐高温相关基因功能的研究。In order to characterize the biological functions of the high-temperature tolerance-related genes of Puccinia striiformis f.sp.tritici（Pst）by using HIGS method,it is necessary to develop a compatible interactive system containing wheat variety and Pst pathogen and Barley stripe mosaic virus（BSMV）.In this study,20 varieties（highly susceptible when inoculated at 10℃）were inoculated with 22 high-temperature-tolerant isolates at 21℃ and their compatibility levels were evaluated,and we found 11 highly susceptible varieties among the tested ones and 3 isolates with a highly stable level of high-temperature tolerance and a wider virulence spectrum.Based on the results,6 varieties such as‘Local Red＇were compatible with the BSMV strain containing aTaPDSfragment.Therefore,the combinations of the variety‘Local Red＇with the isolates like 15335-2 and 15323-4 were the best interactive systems for characterizing high temperature-tolerance-related genes of Pst with BSMV Agro/LIC.

关 键 词：小麦条锈病 基因干涉 大麦条纹花叶病毒 耐高温性 互作体系 

分 类 号：S435.121[农业科学—农业昆虫与害虫防治]