山西省清徐县葡萄霜霉菌对烯酰吗啉的抗药性分析  

Identification of the dimethomorph-resistant isolates of Plasmopara viticola populations in Qingxu,Shanxi Province

作　　者：王喜娜 王敏[2] 孔繁芳[1] 王忠跃[1] 张昊[1] 

机构地区：[1]中国农业科学院植物保护研究所,植物病虫害生物学国家重点实验室,北京100193 [2]山西省农业科学院果树研究所,太原030031

出　　处：《植物保护》2018年第1期139-142,共4页Plant Protection

基　　金：国家公益性行业(农业)科研专项(201203035); 国家现代农业产业技术体系(nycytx-30); 中国农业科学院科技创新工程项目

摘　　要：葡萄霜霉病是葡萄最重要的病害之一,目前防治霜霉病主要依赖化学药剂,其中羧酸酰胺类杀菌剂是一类重要药剂,然而病原菌抗药性对其防效有重要影响。2015年本实验室首次在中国检测到抗烯酰吗啉的葡萄霜霉菌,并开发了Tetra-Primer ARMS PCR快速检测法来检测抗药性。本研究利用此技术对采自山西省清徐县60株葡萄霜霉菌进行了烯酰吗啉抗性检测,结果显示其中1株具有抗药性,其他59株均为敏感菌株,说明该地区霜霉菌已经开始产生烯酰吗啉抗性,但尚未大范围扩展,需要进行持续监测以指导杀菌剂的使用,这也是首次报道山西省葡萄霜霉菌存在烯酰吗啉抗性菌株。Grape downy mildew is one of the most important diseases of grape.Chemical control is the most effective strategy for controlling this disease so far and carboxylic acid amide（CAA）fungicides are the main agents.However,the efficacy of CAAs in the field is limited due to developmental resistance of pathogen.In 2015,we detected dimethomorph-resistant Plasmopara viticola in China for the first time and developed the Tetra-Primer ARMS PCR method for dimethomorph-resistance detection.Based on this method,60 isolates of P.viticola collected from Qingxu County of Shanxi Province were identified.The results showed that one strain was resistant to dimethomorph and the others were all sensitive,indicating the presence of dimethomorph-resistant P.viticolain this region.Although the low resistance frequency suggested that the resistance allele had not spread widely,continuous monitoring is required for the use of fungicides.This is also the first report of dimethomorph-resistant strains of P.viticolain Shanxi.

关 键 词：葡萄霜霉菌 Tetra-Primer ARMS PCR技术 烯酰吗啉 抗药性 

分 类 号：S436.631.1[农业科学—农业昆虫与害虫防治]