受纳米毒性胁迫的亚硝化菌性能恢复  

Recovery performance of nitrosomonas europaea under toxicity of nanoparticles

作　　者：常岩 吴俊康 薛梦婷 吴际萌 戴文 杨新萍[2] 张健 余冉[1] 

机构地区：[1]东南大学能源与环境学院,南京210096 [2]南京农业大学资源与环境学院,南京210095 [3]浙江省环境监测中心,杭州310012

出　　处：《东南大学学报（自然科学版）》2018年第1期92-98,共7页Journal of Southeast University：Natural Science Edition

基　　金：国家自然科学基金资助项目(51678134;51208092;31370539);中央高校基本科研业务费专项资金资助项目(3203007405)

摘　　要：探讨分别受50 mg/L纳米TiO_2(n-TiO_2)和纳米CeO_2(n-Ce O2)及1,10,25,50 mg/L纳米Zn O(n-Zn O)6 h胁迫后的生物脱氮系统毒性敏感指示性亚硝酸菌-Nitrosomonas europaea的生化性能恢复.结果表明,分别受3种50 mg/L纳米颗粒胁迫抑制的N.europaea细菌浓度、氨氧化速率、细胞膜完整性及氨单加氧酶(AMO)等指标均无法得到恢复.除n-Zn O外,受n-TiO_2和n-CeO_2胁迫后菌群在短期(12 h)内仍能够维持其氨氧化活性.受损菌群性能恢复能力与纳米颗粒胁迫剂量呈负相关性.分别受25和50 mg/L n-Zn O毒性影响后的菌群氨氧化速率、AMO活性均持续减弱;受10 mg/L n-Zn O胁迫后的受损菌群仅细菌浓度、细胞膜完整性指标在12 h内维持其受损生化性能;而受1 mg/L n-Zn O胁迫后受损菌群细菌浓度、氨氧化速率、细胞膜完整性及AMO酶活性12 h内则可恢复至与正常菌群无显著差异.因此,受纳米颗粒短期胁迫的细菌在胁迫消除后具有生化性能修复潜能,但恢复程度受胁迫种类和胁迫强度制约.The recovery potentials of the representative ammonia-oxidizing bacteria,Nitrosomonas europaea are investigated after their 6 h exposure to typical metal oxide nanoparticles Ti O2（ n-Ti O2）,or Ce O2（ n-Ce O2） at 50 mg/L,or Zn O nanoparticles（ n-Zn O） at a series of concentrations（ 0,1,10,25,and 50 mg/L）. The experimental results show that the cell density,membrane integrity,ammonia oxidation rate,and ammonia monooxygenase（ AM O） activity can not be recovered after 50 mg/L nanoparticle stress on cells,the impacted cells can maintain their ammonia oxidation activities after 6 h exposure to 50 mg/L n-Ti O2 or n-Ce O2,while those affected by 25 mg/L or50 mg/L n-Zn O cause irretrievable cell damages with ammonia oxidation rate and AM O activity deprived; the 10 mg/L n-Zn O impacted cells only maintained cell density and membrane integrity levels during 12 h incubation period; all the physiological and metabolic activities of 1 mg/L n-Zn O impaired cells are nearly completely restored during a 12 h recovery incubation period and display no significant difference with those of normal cells. Therefore,the cells can recover their physiological and metabolic activities after exposure to metal oxide nanoparticles with their recovery potential directly related to nanoparticle types and concentrations.

关 键 词：纳米颗粒 氨氧化菌 去除胁迫 机能恢复 

分 类 号：X703.5[环境科学与工程—环境工程]