FGF8、SHH、FOXL2基因在鸡胚成纤维细胞中的调控作用研究  

The regulation of FGF8, SHH and FOXL2 in chicken embryonic fibroblast cells

作　　者：张虎军[1] 赵宗胜[2] 翟曼君[2] 郑炜[2] 李青峰[2] Zhang Hujun;Zhao Zongsheng;Zhai Manjun;Zheng Wei;Li Qingfeng(College of Life Sciences, Shihezi University, Shihezi, Xinjiang 832003, China;College of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang 832003, China)

机构地区：[1]石河子大学生命科学学院,新疆石河子832003 [2]石河子大学动物科技学院,新疆石河子832003

出　　处：《石河子大学学报（自然科学版）》2017年第5期625-631,共7页Journal of Shihezi University(Natural Science)

基　　金：国家自然科学基金项目(30660125)

摘　　要：为了研究SHH、FGF8和FOXL2三个基因在鸡和鹌鹑杂交禽胚胎早期发育中的作用,分别构建鸡SHH、FGF8和FOXL2三个基因共9个RNA干扰载体,构建FGF8基因和SHH基因的过表达载体;将干扰载体和过表达载体分别转染鸡胚成纤维细胞。结果表明:FGF8基因通过MAPK信号通路影响杂交种胚胎早期发育;SHH基因通过Hedgehog信号通路影响杂交种胚胎早期发育;FOXL2基因通过调节DMRT1、SOX9和CYP19等基因的表达变化来影响杂交种胚胎早期发育。本研究可为进一步研究雌性杂交种胚胎早期死亡的原因奠定理论基础。To research the function mechanism of SHH, FGF8 and FOXL2 in embryologic development of chicken-quail hybrids. Nine RNA interference vectors of FGF8, SHH, FOXL2 genes, two overexpression vectors of FGF8, SHH genes were constructed successfully, respectively. These vectors were transferred into chicken embryonic fibroblast cells by liposomes transfection, respectively. The results showed that FGF8 maybe influence early embryologic development of hybrid by effecting MAPK signaling pathway; SHH maybe affect embryologic development through having effect on Hedgehog signaling pathway; FOXL2 influence early embryologic development by regulating the expression level of DMRT1, SOX9 and CYP19. The results would build the theory base for further study of death reason during early development of female hybrid embryos.

关 键 词：鸡与鹌鹑杂交 RNA干扰 FGF8基因 SHH基因 FOXL2基因 

分 类 号：S831[农业科学—畜牧学]