miR-155/SOCS1通路协同IL-17调控日本血吸虫早期感染的研究  

The role of miR-155/SOCS1 pathway in collaboration with IL-17 in modulating the early progression of Schistosoma japonica

作　　者：李覃[1] 尤伟杰[2] 葛新[1] 马萍[1] 邢杰[1] LI Tan;YOU Wei-jie;GE Xin;MA Ping;XING Jie(Department of Pathogen Biology, Logistics University of the Chinese People＇s Armed Police Force, Tianjin 300309, China;Editorial Department of Medical Journal of the Chinese People＇s Armed Police Force, Beijing 100039, China)

机构地区：[1]武警后勤学院病原生物学教研室,天津300309 [2]<武警医学>编辑部,北京100039

出　　处：《军事医学》2017年第10期796-799,共4页Military Medical Sciences

基　　金：武警后勤学院基础研究项目(WHJ2016013)

摘　　要：目的初步探讨miR-155/SOCS1协同IL-17在日本血吸虫(Schistosoma japonicum)感染早期病程进展中的作用。方法 BALB/c小鼠腹部经皮感染日本血吸虫尾蚴,分别于第8、14周活杀小鼠,分析小鼠体质量及脾指数的变化,H&E染色观察肝病理形态,实时定量PCR检测miR-155及细胞因子信号抑制蛋白1(SOCS1)的mRNA表达,ELISA检测血清IL-17含量。结果小鼠感染后一般状况差,体质量减轻,脾明显肿大致脾指数显著升高,感染8周肝组织开始有虫卵沉积,伴大量炎性细胞浸润,出现明显的肉芽肿和纤维化病变,模型鼠肝炎症程度随感染进展减轻,肉芽肿减少。血吸虫感染后miR-155的基因表达和血清IL-17含量明显增加,感染14周显著下降但仍高于正常对照组,而SOCS1的mRNA表达持续增加。结论 miR-155可能协同IL-17促进血吸虫感染早期的炎症免疫反应,通过负调控SOCS1参与血吸虫感染的病程进展。Objective To explore the role of microRNA-155（ miR-155）/suppressor of cytokine signaling 1（ SOCS1）pathway combined with IL-17 in modulating the early progression of Schistosoma japonicum. Methods BALB/c mice were infected subcutaneously with cercariae of S. japonicum before being sacrificed at 8 and 14 weeks after infection to collect liver samples for pathological observation by HE staining. The spleens of mice were collected to calculate the spleen index. The level of IL-17 in serum was determined using ELISA. Furthermore,qRT-PCR was used to detect the expression of miR-155 and SOCS1 mRNA in the spleens of mice. Results Compared with normal group,the spleen index in the model group was increased significantly along with the loss of relative body mass. HE staining revealed that the deposition of parasite eggs began to appear in the liver tissue at week 8 post-infection. Meanwhile,there were a large number of inflammatory cells infiltrating to form the eosinophilic granuloma. Over time,the formation of egg granulomas and the infiltration of inflammatory cells were alleviated on week 14 post-infection. The level of IL-17 in serum and the expression of miR-155 in the spleen were peaked on week 8 post-infection,and decreased sharply on week 14,but were still evidently higher than those in normal group. The expression of SOCS1 mRNA was progressively increased both on week 8 and 14.Conclusion The data indicates that miR-155 might cooperate with IL-17 to modulate SOCS1 in the development of S. japonicum infection.

关 键 词：日本血吸虫 免疫应答 白细胞介素17 肉芽肿 微小核糖核酸 基因表达 

分 类 号：R532.21[医药卫生—内科学]