卵巢浆液性腺癌中抑癌基因WWOX和PTEN的表达及临床意义  

Expression and Its Clinical Significance of Tumor Suppressor Gene WWOX and PTEN in Ovarian Serous Adenocarcinoma

作　　者：吴铃 叶健文[1] 林舟[1] 

机构地区：[1]福州市第一医院妇科,福建福州350004

出　　处：《中外医疗》2017年第33期23-24,27,共3页China & Foreign Medical Treatment

摘　　要：目的研究抑癌基因WWOX及PTEN对卵巢浆液性腺癌细胞生长的影响,探讨卵巢癌基因治疗的新靶点。方法于2013年10月—2016年10月福州市第一医院方便选取的60例卵巢浆液性腺癌标本作为研究对象,另选取30例正常卵巢组织作为对照。采用免疫组化检测抑癌基因WWOX及PTEN的阳性率表达,RT-PCR法测WWOX及PTEN的m RNA相对表达量。结果卵巢浆液性腺癌中抑癌基因WWOX及PTEN的免疫组化阳性率及m RNA相对表达量均显著低于正常卵巢组织组(P<0.05)。结论 WWOX及PTEN基因缺失可能是卵巢浆液性腺癌发生的重要分子基础,可能会干扰卵巢癌细胞的细胞周期并抑制其生长增殖,临床研究有待从基因分子调控等方面进一步深入探讨,为卵巢浆液性腺癌的基因治疗提供一种新方法。Objective This paper tries to study the effect of WWOX and PTEN on the growth of ovarian serous adenocarcinoma cells and to explore the new target of ovarian cancer gene therapy. Methods From October 2013 to October 2016, 60 cases of ovarian serous sex adenocarcinoma were selected from fuzhou first hospital,30 other normal ovarian tissue were selected as the control.Immunohistochemistry was used to detect the expression of WWOX and PTEN, the m RNA expression of WWOX and PTEN was measured by RT-PCR.Immunohistochemistry was used to detect the expression of WWOX and PTEN, the m RNA expression of WWOX and PTEN was measured by RT-PCR. Results The immunohistochemical positive rate of m RNA and m RNA expression of WWOX and PTEN in ovarian serous adenocarcinoma were significantly lower than those in normal ovarian tissue（P〈0.05）. Conclusion WWOX and PTEN may interfere with the cell cycle of ovarian cancer cells and inhibit their growth and proliferation, which may provide a new method for gene therapy of ovarian serous adenocarcinoma. WWOX and PTEN gene deletion may be an important molecular basis for the development of ovarian serous adenocarcinoma. Clinical research needs to be further explored in terms of gene molecular regulation.

关 键 词：浆液性腺癌 抑癌基因 WWOX PTEN 

分 类 号：R725[医药卫生—儿科]