大麻素受体1对小鼠脂质代谢的作用研究  

Effect of Cannabinoid Receptor 1 on Lipid Metabolism in Diet-induced Obese Mice

作　　者：魏立雯 袁章琴[2] 赵明德 韩建红 古从伟 付陆 

机构地区：[1]西南医科大学实验动物中心,四川泸州646000 [2]苏州大学附属第一医院骨科研究所,江苏苏州215006

出　　处：《四川动物》2018年第1期51-56,共6页Sichuan Journal of Zoology

基　　金：四川省教育厅基金项目(14ZA0146)

摘　　要：目的研究大麻素受体1(CB1)对高脂饮食诱导肥胖模型小鼠脂质代谢调节作用的机制。方法高脂饮食喂养雄性C57BL/6J小鼠构建肥胖模型小鼠。灌胃给药CB1抑制剂利莫那班(SR141716),观察小鼠体质量、肝脏质量及血清生化指标,检测CB1在皮下脂肪、内脏脂肪、骨骼肌、肝脏、心脏中的mRNA和蛋白质水平,着重探索CB1对肉碱棕榈酰转移酶1(CPT1)基因在mRNA水平表达情况的变化。结果 SR141716抑制了CB1在小鼠各组织中mRNA和蛋白质水平的表达(P<0.05),显著降低了小鼠体质量、肝脏质量(P<0.05),降低了血清总胆固醇、高密度脂蛋白、脂联素和瘦素含量(P<0.05);CB1受抑制后,组织中CPT1A和CPT1B基因mRNA的表达水平明显上调(P<0.05);未检测到CPT1A和CPT1B在心脏的差异表达。结论证实CB1通过作用于CPT1A、CPT1B基因发挥对脂质代谢的调节作用,为靶向调控脂质代谢提供了可靠的依据。Objective To study the regulatory mechanism of cannabinoid receptor 1（ CB1） on the lipid metabolism of diet-induced obese mice. Methods The C57 BL/6 J male mice were feed with high-fat diet,and then the effect of rimonabant on the body mass,liver mass and serum biochemical index of mice were detected. In particular,the mRNA levels of CB1 and carnitine palmitoyltransterase-1（ CPT1）,and protein levels of CB1 in subcutaneous fat,visceral fat,skeletal muscle,liver and heart were also tested. Results It was showed the body mass and liver mass,as well as the mRNA and protein levels of CB1 were all significantly decreased in rimonabant-treated mice（ P 〈 0. 05）. Simultaneously,the levels of total cholesterol,high density lipoprotein,adiponectin and leptin were improved（ P 〈 0. 05）. Furthermore,the mRNA levels of carnitine palmitoyltransterase-1 A（ CPT1 A） and carnitine palmitoyltransterase-1 B（ CPT1 B） in each tissue were increased,while no obviously difference was detected in the expression of CPT1 A and CPT1 B in mouse heart. Conclusion It was suggested that CB1 can regulate lipid metabolism by controlling the expression of CPT1 A and CPT1 B genes. A basis for clinical treatment of lipid metabolism disorders was provided.

关 键 词：大麻素受体1 利莫那班 肉碱棕榈酰转移酶1 肥胖 脂质代谢 

分 类 号：Q95-33[生物学—动物学]