CNCs增强PCL复合纤维支架的静电纺丝制备及其性能表征  

Fabrication and characterization of electro-spun composite fibrous scaffolds for tissue engineering by CNCs-reinforced PCL

作　　者：王金茹 倪晓慧[1] 程万里[1] 韩广萍[1] 

机构地区：[1]东北林业大学材料科学与工程学院生物质材料科学与技术教育部重点实验室,哈尔滨150040

出　　处：《林业工程学报》2018年第1期83-88,共6页Journal of Forestry Engineering

基　　金：国家自然科学基金(31470580);国家林业局引进先进林业科学技术项目(2013-4-11)

摘　　要：采用静电纺丝技术制备了聚己内酯(PCL)纤维支架,并将酸水解制得的纤维素纳米晶体(CNCs)作为增强体,制备了不同CNCs加载量的CNCs/PCL复合纤维支架,以提高PCL纤维支架的力学性能,并探讨了CNCs对PCL纤维支架细胞相容性的影响。结果表明:最佳增强条件是m(CNCs)/m(PCL)为5.25%,在该条件下制备的复合纤维支架(CNCs-5.25/PCL)最大应力和断裂伸长与纯PCL纤维支架相比分别提高了291%和320%。复合纤维支架上成功培养了成人胰腺导管癌细胞(Panc-1)和肝细胞(HL7702);对成人胰腺导管癌细胞在PCL及CNCs/PCL复合纤维支架上的增殖速率进行的单因素方差分析表明,CNCs没有增大PCL复合纤维支架的细胞毒性。CNCs可以作为静电纺PCL纤维支架的增强材料,制备具有良好力学性能和细胞相容性的纳米复合纤维支架,为CNCs在组织工程中的应用提供了理论依据。In order to improve the mechanical properties of polycaprolactone（ PCL） fibrous scaffolds,electro-spun pure fibrous scaffolds and composite fibrous scaffolds at various cellulose nanocrystals（ CNCs） loading levels were prepared. Obtained by acid hydrolysis,the CNCs were used as a reinforcement. The morphology,crystallinity as well as thermal and mechanical properties of CNCs-filled and unfilled fibrous scaffolds were examined by the scanning electron microscopy（ SEM）,wide angle X-ray diffraction（ WXRD）,thermogravimetric analysis（ TG）,and tensile testing. The influences of the CNCs on the cell compatibility of the PCL fibrous scaffolds were also explored. The SEMimages of the obtained fibrous scaffolds demonstrated that the nanofibers were successfully prepared and the average diameter of the fibers for the scaffolds increased slightly and then decreased with the increased CNCs loading in the fibrous scaffold. The thermal stabilities of the CNCs-reinforced fibrous scaffolds were not higher than electro-spun pure PCL fibrous scaffolds. The crystallinity of the fibrous scaffolds also increased firstly and then decreased with the increase in CNCs loading. Furthermore,the optimal reinforcement conditions of the PCL fibrous scaffolds were obtained,which was 5.25% of the mass ratio of m（ CNCs）/m（ PCL）. The mechanical strength and elongation at break of the PCL/CNCs scaffolds at 5.25% CNCs loading increased by 291% and 320%,respectively,compared with the pure PCL fibrous scaffolds. In addition,pancreatic ductal cancer cells（ Panc-1） and hepatic cells（ HL7702） derived from human adult were successfully cultured on the PCL/CNCs composite fibrous scaffolds. The results of one-way variance analysis of the proliferation rate for Panc-1 on PCL and CNCs/PCL composite fiber scaffolds indicated that the CNCs did not increase the cytotoxicity of the PCL composite fibrous scaffolds. The CNCs could be used as a reinforcing material to fabricate nanocomposite fibrous scaffolds with good mechanical

关 键 词：静电纺丝 聚己内酯 纤维素纳米晶体 纤维支架 力学性能 细胞相容性 

分 类 号：TQ353[化学工程]