牡丹花瓣高效液相色谱指纹图谱研究  

HPLC Fingerprints Analysis of Peony Petals

作　　者：华梅[1,2] 原晓龙[1,2] 王毅[1,2] 杨卫[1,2] 陈剑[1,2] 马惠芬[1,2] 呼延丽[1,2] 杨宇明 王娟[1,2] 

机构地区：[1]云南省林业科学院,云南昆明650201 [2]云南省森林植物培育与开发利用重点实验室/国家林业局云南珍稀濒特森林植物保护和繁育实验室,云南昆明650201

出　　处：《西部林业科学》2018年第1期52-58,共7页Journal of West China Forestry Science

基　　金：云南省科技计划(对外科技合作计划-国际科技合作)-滇牡丹重要经济性状研究与综合利用技术引进(2015IA005);云南省应用基础研究计划青年项目-滇牡丹花瓣中花色素的提取;分离及纯化工艺研究(2014FD069);云南省森林植物培养与开发利用重点实验室开放基金;云南省中青年学术技术带头人培养项目(2010CI016)

摘　　要：以不同引种地不同品种的9个新鲜牡丹花瓣为实验材料,采用高效液相色谱法(HPLC)测定9个不同引种地不同品种牡丹花瓣样品中的化学成分。液相色谱条件为Eclipse plus C18色谱柱(4.6×150mm,5μm),流动相为乙腈-1%乙酸梯度洗脱,流速0.8mL/min,检测波长360nm,柱温为35℃。通过中药色谱指纹图谱相似度评价系统(国家药典委员会)进行相似度分析,建立9个样品牡丹花瓣的指纹图谱,为科学评价与有效控制牡丹花瓣产品质量提供新方法。结果表明,本研究建立的分析方法有较好的重复性,9个引种牡丹花瓣样品指纹图谱共有6个主要共有峰,可作为鉴别引种地牡丹花瓣质量的主要依据。In order to establish the peony petals chromatographic fingerprints,the fresh Peony petals collected from different introduction regionalization were detected by high performance liquid chromatography( HPLC). The related chromatographic conditions were as follows: Eclipse plus C18 column( 4. 6×150 mm,5μm),with acetonitrile and 1% acetic acid as mobile phase in gradient mode. The detective wavelength was 360 nm,the flow rate was 0. 8 mL/min and the column temperature was 35℃. Chromatographic fingerprints of peony petals from different introduction region were established based on the analysis of similarities of composition. The results showed that:The method had a good repeatability and reproducibility and a total of 6 main common peaks was found in the fingerprints of peony petals in 9 different samples,which could be used as the main basis for the identification of peony petals products and provide a quality control method for peony petals products.

关 键 词：牡丹花瓣 高效液相色谱 指纹图谱 

分 类 号：S567.15[农业科学—中草药栽培]