断体地龙促进创伤愈合活性成分筛选研究  

Screening Active Components of Wound Healing from Tail-amputated Earthworm

作　　者：段晓杰[1] 杨雨薇 赓迪[1] 罗世林[1] 王雄飞[1] 丁玉婷 郑竹宏 赵仁云 孙毅坤[1] 

机构地区：[1]北京中医药大学,北京102488

出　　处：《中医药学报》2018年第1期5-7,共3页Acta Chinese Medicine and Pharmacology

基　　金：国家自然科学基金面上项目(81373942)

摘　　要：目的:从断体地龙提取液中筛选其促进创伤愈合的活性成分。方法:采用DEAE-Sepharose Fast Flow阴离子交换层析对断体地龙提取液进行分离纯化,通过CCK-8法测定不同洗脱液对NIH3T3细胞增殖的作用,筛选活性较强的组分。结果:离子交换层析梯度洗脱后得到5个洗脱峰。在一定浓度范围内,五种成分对NIH3T3细胞均有促进增殖作用,细胞增殖率随着蛋白浓度增大呈依赖上升趋势。当蛋白浓度为1.5μg/m L时,各组细胞增殖率均达到最大值,其中3号洗脱液作用的细胞增殖率最大。结论:断体地龙提取液经离子交换层析后得到的3号洗脱液促进NIH3T3细胞增殖作用最强,表明其可能含有促进创伤愈合的潜在活性成分,有待进一步研究。Objective: To screen out active component of wound healing from the extracts of tail-amputated earthworm.Methods: The extracts were purified by DEAE-Sepharose Fast Flow ion exchange chromatography. The proliferative effects on NIH3 T3 were detected by Cell Counting Kit-8(CCK-8) to filter the most active eluent. Results: The ion exchange chromatography separated the extracts into five fractions after the gradient elution. The results showed that five eluents could promote the proliferation of NIH3 T3 fibroblasts,and the cell proliferation rates increased with protein concentration. The proliferation rates of five groups reached the peak at the concentration of 1. 5 μg/m L,and the third eluent was regarded as the most active fraction. Conclusion: The third eluent is most effective in promoting effects on proliferation activity of NIH3 T3 cells,it may contain potential active ingredients of wound healing,which needs further study.

关 键 词：断体地龙 DEAE-Sepharose FAST FLOW 创伤愈合 NIH3T3 CCK-8 

分 类 号：R284.2[医药卫生—中药学]