ELISA方法检测猪和羊血清中甲型流感抗体的研究  

Study of Influenza A Antibody Detecting from the Serum of Swine and Goats with ELISA Method

作　　者：王中正 

机构地区：[1]铜陵市农业委员会,安徽铜陵244000

出　　处：《安徽农学通报》2018年第2期84-86,99,共4页Anhui Agricultural Science Bulletin

摘　　要：目的:用ELISA方法检测来自安徽铜陵市、凤阳县及蚌埠市养殖场所采集的91份羊与91份猪的血清中甲型流感阳性抗体,以确定其中阳性血清和其所占总量中的比例。方法:采集的样品在实验室经过冷冻离心机来分离血清,并且用ELISA法对这些血清样品进行检测分析,以此检测猪和羊血清中甲型流感的抗体水平。结果:在91份羊血清样品中检测到3份甲型流感阳性抗体,占血清总数的3.3%;在91份猪血清样品中检测到21份甲型流感阳性抗体,占血清总数的23.1%。结论:说明在安徽地区,甲型流感属部分感染且在猪中的感染率高于羊。Objective：Detecting the influenza A antibody in the serum samples which collected from 91 goats and 91 swine in Tongling,Bengbu and Fengyang of Anhui Province with ELISA method,so as to determine the existenceand prevalence of positive serum in the total.Methods：Serum samples were collected with centrifuging in the labora-tory,and detect the antibody levels of H1 N1 flu in swine and goats＇ serum with ELISA method.Results：3 serum sam-ples were influenza A antibody positive detected from 91 goats serum samples,accounting for 3.3% in the total,while 21 influenza A antibody positive were detected from 91 swine serum samples which account for 23.1%.Conclu-sion：There was some prevalence of influenza A in Anhui Province,and the positive rate of serum in swine was higherthan in goat.

关 键 词：流感病毒 ELISA试剂盒 血清抗体 

分 类 号：S858.9[农业科学—临床兽医学]