蒲公英提取物中总酚酸含量测定方法的优化  

Content Determination of Total Phenolic Acids in Extract of Taraxacum mongolicum

作　　者：张艳 余正勇 耿福能 吴桃清 杨亮 肖怀 赵昱 刘衡 

机构地区：[1]云南省昆虫生物医药研发重点实验室,云南大理671000 [2]药用特种昆虫开发国家地方联合工程研究中心,云南大理671000 [3]中国西南药用昆虫及蛛形类资源开发利用协同创新中心,云南大理671000 [4]四川好医生药业集团,四川成都610000

出　　处：《食品研究与开发》2018年第3期123-126,共4页Food Research and Development

基　　金：国家自然科学基金(81660605);药用特种昆虫开发国家地方联合工程研究中心创新能力建设项目(云发改高技[2015]395);云南省昆虫生物医药研发重点实验室专项经费(2015DG030);云南省2011协同创新中心项目([2012]25);云南省应用基础研究重点项目(2014FA007)

摘　　要：以咖啡酸为对照品,采用普鲁士蓝比色法测定蒲公英提取物中总酚酸的含量并对方法学进行考察和优化。并通过单因素考察方法,确定最佳测定方法:显色剂、缓冲剂十二烷基硫酸钠(sodium dodecyl sulfate,SDS)用量为2.0 mL,盐酸浓度为0.1 mol/L,显色20 min。在最佳测定条件下,测得蒲公英提取物中总酚酸含量为120.5 mg/g。该方法稳定性好,精密度高,咖啡酸在2.0μg/mL^20.0μg/mL呈现良好的线性关系(r=0.999 2),平均回收率为98.86%,RSD为0.84%;结果表明该方法简单易行,稳定可靠,可作为蒲公英提取物中总酚酸含量的测定方法。The content of total phenolic acids in the extract of Taraxacum mongolicum was determined by Prussian blue colorimetry and the methodology was investigated and optimized, with caffeic acid as the reference material.The dosage of chromogenic agent and sodium dodecyl sulfate （SDS）, the concentration of hydrochloric acid and the coloration time were investigated by univariate analyses, the results suggested optimum conditions should be as follows：the dose for both chromogenic agent and SDS was 2.0 mL, the concentration of hydrochloric acid was 0.1 mol/L, with the coloration time was 20 min. Applying above-mentioned optimized condition, the content of total phenolic acid in the extract of Taraxacum mongolicum was 120.5 mg/g. This method was simple, stable and reliable, having a good linearrelation in the range of 2.0 μg/mL-20.0 μg/mL（r=0.999 2） with the average recovery of 98.86 % and RSD of 0.84 %. which is suitable for analysis of total polyphenols.

关 键 词：蒲公英 总酚酸 咖啡酸 含量测定 普鲁士蓝法 

分 类 号：R284.1[医药卫生—中药学]