基于叶片电信号边际谱熵的玉米耐盐碱性无损评价方法  

Nondestructive evaluation method for saline-alkaline tolerance of maize based on marginal spectral entropy of electric signal in leaf

作　　者：刘锴[1] 赵燕燕 习岗[1] 杨运经[3] 杜光源[3] 

机构地区：[1]西安理工大学理学院,西安710054 [2]郑州工业应用技术学院基础教学部,郑州451100 [3]西北农林科技大学理学院,杨凌712100

出　　处：《农业工程学报》2018年第2期197-204,共8页Transactions of the Chinese Society of Agricultural Engineering

基　　金：国家自然科学基金资助项目(31471412;51277151);陕西省教育厅科学研究计划项目(15JK1515)

摘　　要：为了探索能够早期、灵敏、在位和无损检测与评价植物耐盐碱性的方法,将Na Cl、Na2SO4、Na HCO3和Na2CO3配置成复合盐碱溶液对耐盐碱性较弱的玉米品种郑单958和耐盐碱性较强的玉米品种名玉20的玉米幼苗进行盐碱胁迫,采集了盐碱胁迫过程中郑单958和名玉20幼苗叶片电信号,应用HHT(Hilbert-Huang transformation)方法得到了2种玉米叶片电信号的边际谱,分析了盐碱胁迫过程中2个玉米品种叶片电信号边际谱熵MSE(marginal spectrum entropy)变化的差异和生物学意义。结果显示:盐碱胁迫过程中,郑单958叶片电信号的MSE表现出不断下降的趋势,叶片中丙二醛MDA(malondialdehyde)含量迅速升高;名玉20的MSE表现出波动性的变化,MDA含量变化不大,表明郑单958叶片细胞的离子跨膜运输被抑制,名玉20的叶片细胞存在着复杂的代谢调节,盐碱胁迫造成的叶片细胞膜脂过氧化可能是叶片电信号MSE变化的原因。研究发现,盐碱胁迫下耐盐碱性不同的2个玉米品种的叶片电信号响应指数RI(response index)差异明显,在胁迫2、3和4 d时郑单958的RI值分别比名玉20增长了42%、193%和332%。根据RI值的大小有可能对盐碱胁迫下玉米叶片细胞离子运输和细胞膜伤害的影响程度进行灵敏和早期的定量诊断,进而实现对玉米幼苗期耐盐碱性强弱的在位和无损伤的评价。Saline-alkaline stress（SAS） is one of the major abiotic stresses affecting the growth of plants.It has been a severe problem that restricts plant production and even the development of the ecological environment.The improvement of plant saline-alkaline tolerance and selection of saline-alkaline tolerance plant varieties are becoming hot spots for research.To develop and select saline-alkaline tolerance plants,an evaluation method that can accurately judge the plant saline-alkaline tolerance must be first established.In the present study,the evaluation of saline-alkaline tolerance of plants is generally based on morphological indicators and physiological and biochemical indicators.These evaluation methods require a large number of samples and long cycle,and cannot be early diagnosed.Moreover,many of the indicators must be obtained through the destructive measurement of test-tube experiments,which are not nondestructive testing.Therefore,the traditional evaluation method has many disadvantages.In order to explore the methods of early,sensitive,in situ and nondestructive testing saline-alkaline tolerance of plants,a complex solution consisting of NaCl,Na2SO4,NaHCO3 and Na2CO3 with pH value of 9.09 was used to stress 2 kinds of maize variety seedlings of Zhengdan 958 with poor saline-alkaline tolerance and Mingyu 20 with strong saline-alkaline tolerance.Time-domain waveforms of leaf electrical signals of Zhengdan 958 and Mingyu 20 seedlings during saline-alkaline stress were collected.The marginal spectra of 2 kinds of maize leaf electrical signals were obtained by Hilbert-Huang transformation（HHT）.The changes of marginal spectrum entropy（MSE） of maize leaf electrical signals and the biological significance were analyzed.The results showed that the MSE of leaf electrical signals from maize variety Zhengdan 958 continued to decline in the process of saline-alkaline stress,while the MSE of leaf electrical signals from maize variety Mingyu 20 changed in volatility.It was indicated that the ion transport of l

关 键 词：胁迫 作物 无损检测 玉米 盐碱胁迫 电信号 边际谱熵 评价 

分 类 号：Q64[生物学—生物物理学]