进水pH值对CASS工艺处理低浓度生活污水中氧化亚氮排放的影响  

Effect of Influent pH Value on N_2O Emission from CASS Process for Low Concentrated Domestic Wastewater Treatment

作　　者：毛念佳 嵇鸿民 许柯[1] 任洪强[1] 李颖瑜 

机构地区：[1]南京大学环境学院污染控制与资源化研究国家重点实验室,江苏南京210046

出　　处：《净水技术》2018年第2期24-30,共7页Water Purification Technology

基　　金：国家科技支撑计划项目(2014BAC08B00)

摘　　要：考察进水pH对CASS工艺处理低浓度生活污水中N_2O排放的影响,利用实时荧光定量PCR和16S rRNA高通量测序分析其机理。结果表明:随着进水pH值由6.6提高到9.0,氨氮去除率由39.5%提高到99.7%,总氮去除率由37.5%提高到45.7%;同时,单周期N_2O排放量从0.32 mg N/L增加到0.52 mg N/L。高进水pH值(8.4和9.0)下,反硝化功能基因nosZ、nirS和nirK丰度较高。微生物群落分析表明,随着进水pH的增加,氨氧化菌含量变化不大,而亚硝酸盐氧化菌含量则从2.58%增加至3.42%。反硝化优势菌种为Dechloromonas(1.36%~4.25%)和Zoogloea(0.75%~1.26%),Dechloromonas丰度随进水pH变化较大。Effect of influent pH value on N2O emission from CASS process for low-concentrated domestic wastewater treatment and its mechanism were studied with real-time qPCR and high-throughput 16S rRNA sequencing. The results showed that NH4 removal rate increased from 39. 5% to 99. 7% when influent pH value was improved from 6. 6 to 9. 0, with the improvement of TN removal rate （from 37. 5% to 45. 7%）. Meanwhile,N2O emission increased from 0. 32 mg N/L to 0. 52 mg N/L in a single cycle. Besides, the abundances of denitrifying funcational genes （nosZ、iirS and nirK） were relatively higher under high influent pH value （ 8. 4 and 9. 0 ） . Microbial structure analysis indicated that ammonium-oxidizing bacteria （ AAOB） didn’t change significantly,while nitrite-oxidizing bacteria （NOB） varied from 2. 58% to 3. 42% in reactors. Dominant denitrifying bacteria in reactors was Dechloromonas （1. 36%？4. 25%） and Zoogloea （0.75% - 1.26%）,dramatic abundance variation of Dechloromonas could be observed under different influent pH value.

关 键 词：氧化亚氮 循环式活性污泥工艺(CASS) 生物脱氮 16S rRNA高通量测序 

分 类 号：TU992.3[建筑科学—市政工程]