PFT-α对鸡传染性喉气管炎病毒侵染宿主细胞的影响  

Effect of PFT-α on Infectious Laryngotracheitis Virus Infecting Host Cells

作　　者：孙邦耀 邵昱昊[1] 高琦[1] 李海[1] 刘胜旺[1] 

机构地区：[1]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室/禽呼吸道病创新团队,黑龙江哈尔滨150069

出　　处：《安徽农业科学》2018年第5期87-90,共4页Journal of Anhui Agricultural Sciences

基　　金：现代农业蛋鸡产业技术体系项目(CARS-41-K12);国家科技支撑计划项目(2015BAD12B03)

摘　　要：[目的]研究抑癌基因p53小分子抑制剂PFT-α对ILTV侵染宿主细胞的影响。[方法]用PFT-α处理LMH(鸡肝癌细胞),通过结晶紫染色技术检测细胞感染ILTV 24 h后的细胞死亡情况,通过流式细胞术检测PFT-α对ILTV吸附、进入LMH细胞的影响,通过倒置荧光显微镜和高内涵细胞筛选系统检测PFT-α对ILTV在细胞间扩散的影响。[结果]结晶紫染色表明,PFT-α促进了ILTV感染后的细胞死亡,流式细胞术结果表明,PFT-α对ILTV吸附细胞没有影响,但是促进了ILTV进入细胞。同时,倒置荧光显微镜和高内涵细胞筛选系统检测结果表明,PFT-α对ILTV在细胞间扩散没有影响。[结论]研究初步检测了PFT-α对ILTV侵染LMH细胞过程的影响,为进一步研究ILTV与宿主互作过程提供了理论补充。[ Objective]To research the effect of PFT-α, a p53 inhibitor, on laryngotracheitis virus（ ILTV） infecting host cells. [ Method] In present study, we detected the effect of PFT-α on cell death, ILTV binding and penetration as well as transmission between LMH cells by stained with 0. 1% crystal violet, flow cytometry and observing expression of EGFP using fluorescence microscopy and Operetta CLS high-con-tent analysis system. [ Result] Crystal violet staining result showed that PFT-α promoted LMH cells death after ILTV infection. Flow cytometry results showed that PFT-α had no effect on viral binding but promoted penetration of ILTV. Results of both fluorescence microscopy observation and Operetta CLS high-content analysis system showed that PFT-α had no effect on ILTV transmission between LMH cells. [ Conclusion] Our findings have advanced our understanding about how ILTV infection host cells and provided another promising option for controlling AILT.

关 键 词：传染性喉气管炎病毒 病毒吸附 病毒进入 病毒扩散 

分 类 号：S852.65[农业科学—基础兽医学]