补肾养血颗粒的提取工艺优化研究  

Study on Optimization of the Extraction Technology of Bushen Yangxue Granules

作　　者：刘霞[1] 杨敏[1] 冷静[1] 范智琴 徐冲[1] 

机构地区：[1]重庆市中医院药剂科,重庆400021

出　　处：《中国药房》2018年第3期349-352,共4页China Pharmacy

基　　金：重庆市科学技术委员会基本科研业务费计划项目(No.cstc2015jbky330025009);中药炮制技术传承基地建设项目

摘　　要：目的:优化补肾养血颗粒的提取工艺,为其开发成医院制剂提供依据。方法:以金丝桃苷含量、总黄酮含量和出膏率的综合评分为评价指标,以加水量、提取时间和提取次数为考察因素,设计正交试验优化补肾养血颗粒的提取工艺并进行验证。结果:补肾养血颗粒最优提取工艺为每次加水10倍量,提取2次,每次1 h。验证试验结果显示,金丝桃苷、总黄酮含量分别为0.154、11.094 mg/g(RSD=0.010%、0.008%,n=3);出膏率均值为37.76%(RSD=0.008%,n=3)。结论:优化后的提取工艺稳定、可行,可为补肾养血颗粒的制剂开发提供依据。OBJECTIVE：To optimize the extraction technology of Bushen yangxue granules,and to provide reference for developing hospital preparations. METHODS：The orthogonal test was designed to optimize the extraction technology of Bushen yangxue granules using the comprehensive score including the content of hyperoside and total flavonoids, extract yield as evaluation indexes,water amount,extraction time and extraction times as factors. Verification test was also conducted. RESULTS：The extraction technology of Bushen yangxue granules was 10-fold water,extracting for 2 times and 1 hour each time. The results of verification test indicated that the contents of hyperoside and total flavonoids were 0.154 mg/g（RSD=0.010%,n=3） and11.094 mg/g（RSD=0.008%,n=3）. The average extract yield was 37.76%（RSD=0.008%,n=3）. CONCLUSIONS：The optimized extraction technology is stable and feasible,which can provide the basis for developing hospital preparations of Bushen yangxue granules.

关 键 词：补肾养血颗粒 提取工艺 金丝桃苷 总黄酮 正交试验 

分 类 号：R284.2[医药卫生—中药学]