银杏外种皮多糖抑制J亚群禽白血病病毒在DF-1细胞中的复制  

ANTIVIRAL ACTIVITY OF GINKGO BILOBA EXOCARP POLYSACCHARIDE AGAINST SUBGROUP J AVIAN LEUKOSIS VIRUS IN DF-1 CELL

作　　者：钱琨 孔正茹[1,2] 张杰 秦爱建[1,2,3] 

机构地区：[1]扬州大学教育部禽类预防医学重点实验室,扬州225009 [2]扬州大学江苏省动物预防医学重点实验室,扬州225009 [3]江苏省动物重要疫病与人兽共患病防控协同创新中心,扬州225009

出　　处：《中国动物传染病学报》2018年第1期13-18,共6页Chinese Journal of Animal Infectious Diseases

基　　金：国家重点研发计划(2017YFD0500700;2016YFD0500800);国家自然科学基金(31772734);江苏省优势学科项目;扬州大学新世纪人才项目

摘　　要：为研究银杏外种皮多糖抑制J亚群禽白血病病毒(Avian leukosis virus subgroup J,ALV-J)复制的能力,在不同时间点加入银杏外种皮多糖处理DF-1细胞,利用q RT-PCR、免疫蛋白印迹以及病毒滴度检测等方法,观察银杏外种皮多糖对ALV-J复制的影响,结果表明银杏外种皮多糖就能够显著地抑制病毒基因表达、蛋白合成以及病毒在细胞内的增殖,并且这种抑制作用具有时间和剂量依赖性。同时银杏外种皮多糖处理还明显抑制病毒感染细胞中的IL-1β、TNFα等细胞因子的表达。银杏外种皮多糖在DF-1细胞中具有抑制ALV-J复制的能力,具有作为抗ALV-J药物的潜力。To investigate the antiviral effects of ginkgo biloba exocarp polysaccharide （GBEP） on the replication of Avian leukosis virus subgroup J （ALV-J）, DF-1 cells were treated with GBEP at different time points. The results indicated that GBEP strongly inhibited viral gene transcription, protein expression and virus titers through dose and time dependent manners as detected using qRT-PCR, Western blot and TCID50 assays. We also noted that GBEP greatly down-regulated the expression of IL-1β, TNFα and other immune related cytokines. Overall, this study highlighted that GBEP exerted its anti-avian leukosis virus activity and might be developed as a preventive drug for ALV-J infection in the future.

关 键 词：J亚群禽白血病病毒 银杏外种皮多糖 病毒复制 抑制 

分 类 号：S852.659.3[农业科学—基础兽医学]