过氧乙酸-乙醇联合辐照灭菌对脱钙骨基质成骨诱导活性的影响  

Effects of peracetic acid-ethanol combined irradiation sterilization on the osteogenic inducing activity of demineralized bone matrix

作　　者：李雄涛[1] 余国荣[2] 余黎[2] 张旗[2] 邓凯[2] 

机构地区：[1]华中科技大学同济医学院附属武汉儿童医院骨科,湖北武汉430016 [2]武汉大学中南医院骨科,湖北武汉430071

出　　处：《生物骨科材料与临床研究》2018年第1期1-4,81,共5页Orthopaedic Biomechanics Materials and Clinical Study

基　　金：武汉市科技攻关项目(201161038344-05)

摘　　要：目的探讨过氧乙酸-乙醇(PES)联合辐照灭菌对脱钙骨基质(DBM)成骨诱导活性的影响。方法取SD大鼠长管骨制作成骨粉装入胶囊后分为两组进行消毒灭活处理:PES+20k Gy的钴-60射线消毒组(实验组)和20k Gy钴-60射线消毒组(对照组)。将DBM骨胶囊植入40只SD大鼠体内,分别在2、4、6、8周处死SD大鼠,将之前植入的DBM骨胶囊取出,对DBM的骨诱导活性进行检测。结果从大体观察看,实验组所植DBM骨块呈散在颗粒状、形态不完整,对照组所植DBM骨块呈卵圆形、质地较硬、形态完整;从新生微血管数(MVQ)看,实验组MVQ值少于对照组,差异有统计学意义(<0.0001);从新生骨生长速率看,对照组的生长速率为1.59 m/d,而实验组生长速率过低,无法测量;从钙、磷、碱性磷酸酶含量看,实验组钙含量小于对照组,差异有统计学意义(<0.05),实验组磷含量小于对照组,差异有统计学意义(<0.001),实验组碱性磷酸酶含量小于对照组,差异有统计学意义(<0.05)。结论使用PES联合辐照灭菌以后会降低DBM的成骨诱导活性。Objective To investigate the osteogenic activity of demineralized bone matrix （DBM） after Peracetic Acid -Ethanol （PES） combined irradiation sterilization. Methods The long bone of SD rats were divided into two groups to disinfection and inactivation the experimental group which used PES solution add the cobalt-60y radiation at 20kGy, and the control group just only used cobalt-60y radiation at 20kGy. We made the DBM bone meal into capsules after di-sinfection, and implanted into SD rats, and the SD rats were sacrificed at 2, 4,6,8 weeks, and remove the plant DBM bone from SD rat to detect the bone induction activity. Results From general observation, the DBM bone fragments from the experimental group were planted incomplete, scattered particles, on the contrary, experimental group disinfection planting had hard texture and complete shape. From new bone quantitative analysis, the results shows that the control group sterilized osteogenic activity is much better than the experimental group. From analysis of the sample of calcium, phosphorus, alkaline phosphatase enzyme content, the control group content is more than experimental group. Conclusion The osteogenic induction activity of DBM was reduced by using PES combined irradiation sterilization.

关 键 词：骨植入材料 过氧乙酸-乙醇 脱钙骨基质 成骨诱导活性 

分 类 号：R318.08[医药卫生—生物医学工程]