ITS克隆测序揭示中国沿海表层水中真核浮游生物多样性  

Biodiversity of Planktonic Eukaryotes in Surface Water of Chinese Coastal Seas Revealed by ITS Sequencing

作　　者：于淑贤[1,2] 李文军 李楠[3] 李富超 王寅初[1] 李佳霖[1] 秦松[1] 

机构地区：[1]中国科学院烟台海岸带研究所海岸带生物与生物资源利用所重点实验室,山东烟台264003 [2]中国科学院大学,北京100049 [3]广西师范学院北部湾环境演变与资源利用教育部重点实验室,广西南宁530001 [4]中国科学院海洋研究所科研处,山东青岛266071

出　　处：《海洋科学进展》2018年第1期108-116,共9页Advances in Marine Science

基　　金：中国科学院战略试点项目--热带西太平洋海洋系统物质能量交换及其影响(XDA1102040300);国家自然科学基金项目--海洋藻红蛋白适应弱光的分子机制(41176144);高光和氮缺乏胁迫下十个雨生红球藻虾青素合成相关基因顺式作用元件的协同作用(41376139)

摘　　要：表层水体中的浮游生物群落在生物地球化学过程中起重要作用,其生物地理分布通常与水文等环境条件相关。近年来DNA测序等方法已成为研究海洋生态系统中真核生物群落的重要工具,在本研究中采用ITS1作为DNA标记评估我国沿海表层水中的真核浮游生物多样性。BLAST结果表明,大部分ITS1序列隶属于6个真核类群,包括浮游动物、真菌和囊泡虫类等。这些主要的真核生物类群可以将采样点分为显著不同的5个聚类,而每个聚类中的特征真核生物门类表现出显著的地理分布差异。总体上,真核浮游生物群落的多样性水平与经纬度、水深、温盐等参数显著相关,自北向南有显著升高的趋势。真核生物群落差异(β-多样性)随地理距离的增加而加大。这提示真核浮游生物在我国沿海表层水中显示出地理限制。本文为中国沿海浮游生物的生物地理研究提供了分子生物学数据基础。Planktonic communities in surface water play an important roles in the biogeochemical processes,and the biogeographic distribution is usually related to the environmental conditions such as hydrology.Recently,molecular approaches have become major solutions to investigate eukaryotic community in the marine ecosystem.In this study,ITS1 was used as a DNA marker to estimate diversity of eukaryotic plankton in the surface water of the Chinese coastal waters.Results of BLAST indicated that most of the ITS1 sequences were affiliated with six eukaryotic groups,including Metazoa,Fungi and Alveolata.The abundant eukaryotic plankton could classify the sampling sites into five distinct clusters.For each cluster,the dominant phyla exhibited significant geographic variation.Generally,diversity of eukaryotic plankton was significantly related to longitude,latitude,depth,temperature and salinity,presenting ageneral increasing tendency from the north to south of coastal seas.Community dissimilarity of eukaryotes(β-diversity)increased with the increasing geographic distance.These results indicate that the eukaryotic plankton were subject to the geographical limits in the surface water of the Chinese coastal seas.Hereby this study provides molecular data for the biogeographic distribution of eukaryotic plankton along the coast of China.

关 键 词：真核浮游生物 中国沿海 ITS 生物多样性 

分 类 号：Q178[生物学—水生生物学]