双孢蘑菇不同交配型同核不育单孢的基因组文库构建  

Genomic Library Construction of Monokaryons with Different Mating Type of Agaricus bisporus

作　　者：蔡志欣[1] 陈美元[1] 廖剑华[1] 李洪荣[1] 郭仲杰[1] 王泽生[1] 

机构地区：[1]福建省农业科学院食用菌研究所/特色食用菌繁育与栽培国家地方联合工程研究中心,福建福州350014

出　　处：《福建农业学报》2017年第12期1327-1331,共5页Fujian Journal of Agricultural Sciences

基　　金：福建省科技计划项目--省属公益类科研院所基本科研专项(2015R1020-1);国家现代农业产业技术体系建设专项(CARS20);福建省财政专项--福建省农业科学院科技创新团队建设项目(STIT2017-1-6);福建省种业创新产业化工程项目(fjzycxny2017009)

摘　　要：对24个双孢蘑菇不育单孢进行两两杂交配对,以菌株9608为基准,获得了2种不同交配型的不育单孢菌株A+:9601、9608、9609、9612、AgQG841-1、AgLH830-4、AgLH830-6、AgQL8125-5;A-:9602、9603、9604、9607、Ag2k811-1、M7206-1、M7206-2、M7206-11、M7206-13。将这2种菌株进行DNA提取,并构建DNA文库,对文库进行酶切验证,用SalⅠ酶切,可切出20、14、9kb等3个片段,其中20、9kb为λ载体的左右臂,插入片段为14kb,与预期相符,说明文库的质量较高。In this study,twenty-two Agaricus bisporus monokaryons strains were pairwisely paired.Based on strain 9608,two different mating types of monokaryons were obtained.A + strains:9601,9608,9609,9612,AgQG841-1,AgLH830-4,AgLH830-6,AgQL8125-5.A-:9602,9603,9604,9607,Ag2k811-1,M7206-1,M7206-2,M7206-11,M7206-13.The genomic DNA of two strains were extracted and DNA library were constructed.The library was verified by restriction enzyme digestion with SalI.The 20 kb,14kb and 9kb 3fragments were cut out,of which 20 kb and 9kb were the inserted into the left and right arms of lambda vector and the fragment of 14 kb,was the insertion genomic DNA fragments.Enzyme digestion result was in line with expectations,indicating the higher quality of the library.

关 键 词：双孢蘑菇 不育单孢 交配型因子 基因组文库 

分 类 号：S646.1[农业科学—蔬菜学]